Department of Obstetrics and Gynecology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, PR China.
Oncol Rep. 2012 May;27(5):1353-9. doi: 10.3892/or.2012.1668. Epub 2012 Feb 1.
The target protein for Xklp2 (TPX2), a microtubule-associated protein, can be used to evaluate more precisely the proliferative behavior of tumor cells. The abnormal expression of TPX2 in various types of malignant tumors has been reported, but less is known for cervical cancer. We studied the relationship between TPX2 expression and the biological behavior of cervical cancer. Immunohistochemistry and RT-PCR were used to detect the expression of TPX2 in cervical cancer tissues. The inhibitory effect of TPX2-siRNA on the growth of HeLa human cervical carcinoma cells was studied in vitro. TPX2 expression was found to be significantly higher in cervical carcinoma compared to normal cervical tissues and CIN. The expression of TPX2 in cervical cancer was correlated with histological grading, FIGO staging and lymph node metastasis. TPX2 RNAi in HeLa cervical cancer cells caused S-phase cell cycle arrest, induced apoptosis and inhibited cell proliferation and invasion. In conclusion, TPX2 shows potential to be used as a new marker for cervical cancer diagnosis and therapy.
Xklp2（TPX2）的靶蛋白是一种微管相关蛋白，可用于更精确地评估肿瘤细胞的增殖行为。已报道TPX2 在各种类型的恶性肿瘤中的异常表达，但对宫颈癌的了解较少。我们研究了 TPX2 表达与宫颈癌生物学行为之间的关系。采用免疫组织化学和 RT-PCR 检测宫颈癌组织中 TPX2 的表达。在体外研究了 TPX2-siRNA 对 HeLa 人宫颈癌细胞生长的抑制作用。与正常宫颈组织和 CIN 相比，宫颈癌中 TPX2 的表达明显升高。TPX2 在宫颈癌中的表达与组织学分级、FIGO 分期和淋巴结转移相关。TPX2 RNAi 可使 HeLa 宫颈癌细胞的 S 期细胞周期停滞，诱导细胞凋亡，并抑制细胞增殖和侵袭。总之，TPX2 有望成为宫颈癌诊断和治疗的新标志物。
