静电相互作用驱动假单胞菌噬菌体ϕ6 衣壳的自组装和转录活性。
Electrostatic interactions drive the self-assembly and the transcription activity of the Pseudomonas phage ϕ6 procapsid.
机构信息
Department of Biosciences, University of Helsinki, Vikki Biocenter, Helsinki, Finland Institute of Biotechnology, University of Helsinki, Viikki Biocenter, Helsinki, Finland.
Department of Biosciences, University of Helsinki, Vikki Biocenter, Helsinki, Finland
出版信息
J Virol. 2014 Jun;88(12):7112-6. doi: 10.1128/JVI.00467-14. Epub 2014 Apr 9.
Abstract
Assembly of an empty procapsid is a crucial step in the formation of many complex viruses. Here, we used the self-assembly system of the double-stranded RNA bacteriophage ϕ6 to study the role of electrostatic interactions in a scaffolding-independent procapsid assembly pathway. We demonstrate that ϕ6 procapsid assembly is sensitive to salt at both the nucleation and postnucleation steps. Furthermore, we observed that the salt sensitivity of ϕ6 procapsid-directed transcription is reversible.
摘要
空衣壳的组装是许多复杂病毒形成的关键步骤。在这里，我们使用双链 RNA 噬菌体 φ6 的自组装系统来研究静电相互作用在无支架衣壳组装途径中的作用。我们证明了 φ6 衣壳的组装对成核和成核后步骤中的盐都很敏感。此外，我们观察到 φ6 衣壳指导的转录的盐敏感性是可逆的。
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