噬菌体 φ6 衣壳表面蛋白 8 与含有主要包膜蛋白 9 的病毒特异性膜泡相互作用。
Bacteriophage ϕ6 nucleocapsid surface protein 8 interacts with virus-specific membrane vesicles containing major envelope protein 9.
机构信息
Department of Biosciences, University of Helsinki, Biocenter 2, Helsinki, Finland.
出版信息
J Virol. 2012 May;86(9):5376-9. doi: 10.1128/JVI.00172-12. Epub 2012 Feb 29.
Abstract
Enveloped double-stranded RNA (dsRNA) bacterial virus Pseudomonas phage ϕ6 has been developed into an advanced assembly system where purified virion proteins and genome segments self-assemble into infectious viral particles, inferring the assembly pathway. The most intriguing step is the membrane assembly occurring inside the bacterial cell. Here, we demonstrate that the middle virion shell, made of protein 8, associates with the expanded viral core particle and the virus-specific membrane vesicle.
摘要
包膜双链 RNA (dsRNA) 细菌病毒假单胞菌噬菌体 ϕ6 已被开发成为一种高级组装系统，其中纯化的病毒蛋白和基因组片段可自行组装成感染性病毒颗粒，推断出组装途径。最有趣的步骤是在细菌细胞内发生的膜组装。在这里，我们证明了由蛋白 8 组成的中间病毒壳与扩展的病毒核心颗粒和病毒特异性膜囊泡结合。
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