Ishizuka J, Asada I, Poston G J, Lluis F, Tatemoto K, Greeley G H, Thompson J C
Third Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Pancreas. 1989;4(3):277-81. doi: 10.1097/00006676-198906000-00001.
Pancreastatin is a novel peptide that was recently purified from extracts of the porcine pancreas. The present study shows that pancreastatin (10(-9)-10(-8) M) can stimulate release of insulin from both the isolated perfused rat pancreas and from cultured rat islet cells in the presence of a low, non-insulinotropic concentration of glucose (4.2 mM). Pancreastatin (10(-9) M) can also inhibit release of insulin stimulated by a high concentration of glucose (16.7 mM). Pancreastatin, at 10(-8) M, can enhance glucose (8.3 mM) induced release of insulin in the static islet cell incubation. In addition, pancreastatin (10(-9)-10(-8) M) can inhibit, in a dose-dependent fashion, cholecystokinin (CCK)-8 stimulated release of amylase from dispersed guinea pig pancreatic acini. Pancreastatin alone, however, did not affect basal release of amylase. Our study shows that pancreastatin can exert a direct effect on both pancreatic endocrine and exocrine secretion.
胰抑制素是一种最近从猪胰腺提取物中纯化出来的新型肽。本研究表明，在低浓度、无促胰岛素作用的葡萄糖（4.2 mM）存在的情况下，胰抑制素（10⁻⁹ - 10⁻⁸ M）能够刺激分离灌注的大鼠胰腺以及培养的大鼠胰岛细胞释放胰岛素。胰抑制素（10⁻⁹ M）也能够抑制高浓度葡萄糖（16.7 mM）刺激的胰岛素释放。在静态胰岛细胞孵育中，10⁻⁸ M的胰抑制素能够增强葡萄糖（8.3 mM）诱导的胰岛素释放。此外，胰抑制素（10⁻⁹ - 10⁻⁸ M）能够以剂量依赖的方式抑制胆囊收缩素（CCK）-8刺激分散的豚鼠胰腺腺泡释放淀粉酶。然而，单独的胰抑制素并不影响淀粉酶的基础释放。我们的研究表明，胰抑制素能够对胰腺内分泌和外分泌分泌产生直接影响。
