Naderi Mohammad, Yaghootkar Hanieh, Tara Fatemeh, Tavakkol Afshari Jalil, Farid Hosseini Reza, Ghayour Mobarhan Majid, Shapouri Moghadam Abbas, Mirteimouri Masoumeh, Tara Seyedeh Maryam
Mashhad University of Medical Sciences, Mashhad, IR Iran.
Genetics of Complex Traits, University of Exeter, Heavitree Rd, Exeter, United Kingdom.
Iran Red Crescent Med J. 2014 Jan;16(1):e11195. doi: 10.5812/ircmj.11195. Epub 2014 Jan 5.
Preeclampsia is the most common serious disorder during pregnancy and studies show several immune-related processes in its pathophysiology. The role of cytokines and their expression remains controversial in this field. One of the cytokines of interest in recent studies has been TNF-α, which has been shown to have a higher level in maternal plasma of preeclamptic women.
This study was designed to evaluate the role of TNF-α polymorphism at position -238 in the risk of developing preeclampsia during pregnancy.
One hundred fifty three preeclamptic cases and 140 healthy pregnant women were retrieved from two major hospitals of Mashhad, Iran. Methods a case-control study were designed. Anyone with a history of inflammatory disease, hypertension, or chronic kidney disease was excluded. DNA was extracted from peripheral blood leukocytes. Both groups were genotyped for the polymorphism of the TNF-α gene at position -238 by the RFLP method with Ava II enzyme. Allele and genotype frequencies were compared using one-way ANOVA and the Fisher's exact test.
There were significant differences between the two groups in TNF-α genotype at position -238 (P < 0.001). In the preeclamptic group, the frequency of the AA genotype was higher (P < 0.001) and the frequency of the GG genotype was lower (P < 0.001). The overall prevalence of the A allele at position -238 was higher in preeclamptic cases (P < 0.001).
In this study group, TNF-α -238 polymorphism was shown to be different in preeclamptic and non-preeclamptic pregnant women. The AA genotype and the A allele may carry an increased risk for developing of preeclampsia.
子痫前期是孕期最常见的严重疾病，研究表明其病理生理学涉及多个免疫相关过程。细胞因子及其表达在该领域的作用仍存在争议。近期研究中关注的细胞因子之一是肿瘤坏死因子-α（TNF-α），子痫前期女性母体血浆中该因子水平较高。
本研究旨在评估TNF-α基因-238位点多态性在孕期发生子痫前期风险中的作用。
从伊朗马什哈德的两家主要医院选取了153例子痫前期病例和140例健康孕妇。设计了一项病例对照研究。排除有炎症性疾病、高血压或慢性肾病病史的任何人。从外周血白细胞中提取DNA。采用Ava II酶切的限制性片段长度多态性（RFLP）方法对两组进行TNF-α基因-238位点多态性基因分型。使用单向方差分析和Fisher精确检验比较等位基因和基因型频率。
两组在TNF-α基因-238位点的基因型存在显著差异（P < 0.001）。子痫前期组中，AA基因型频率较高（P < 0.001），GG基因型频率较低（P < 0.001）。子痫前期病例中-238位点A等位基因的总体患病率较高（P < 0.001）。
在本研究组中，子痫前期和非子痫前期孕妇的TNF-α -238多态性存在差异。AA基因型和A等位基因可能增加子痫前期发生的风险。
