Cheng Yuting, An Baiquan, Jiang Man, Xin Yongning, Xuan Shiying
Department of Gastroenterology, Qingdao Medical College; Nanjing Medical University, Qingdao, China.
Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao Medical College, Nanjing Medical University, Qingdao, China.
Hepat Mon. 2015 Mar 31;15(3):e26818. doi: 10.5812/hepatmon.26818. eCollection 2015 Mar.
Cardiovascular events account for the main cause of death in patients with non-alcoholic fatty liver disease (NAFLD), and are largely influenced by genetic factors. Although multiple studies showed that tumor necrosis factor-alpha (TNF-α) polymorphisms are risk factors in the progression of NAFLD, few papers on the association of the polymorphisms and the developing coronary artery disease (CAD) in NAFLD patients have been reported.
The present study was designed to evaluate the association of TNF-α polymorphisms at residues -238 and -308, with the risk of developing CAD in Chinese patients with NAFLD.
The TNF-α polymorphisms at residues 238 and 308 were genotyped in B-type ultrasonography proven NAFLD patients with (n = 246), without (n = 247) CAD and healthy controls (n = 304), using polymerase chain reaction (PCR). Serum lipid profiles were determined using biochemical methods. Statistical analyses were performed using SPSS statistical software, version 20.0 for Mac.
We found a significant association between TNF-α-238 guanine to alanine (GA) polymorphism and carriers of variant allele A between NAFLD patients with and without CAD (P < 0.05). Carriers of the A allele of TNF-α-238 had higher serum triglycerides (TG) and low density lipoprotein (LDL) levels in NAFLD patients with CAD (P = 0.025 and 0.001, respectively) and a higher TG level in NAFLD patients without CAD (P = 0.017), than their non-carrier counterparts.
In the Chinese Han population that we studied, NAFLD patients who carry the TNF-α-238 GA polymorphism have an increased risk of developing CAD. Mechanisms underlying this potentially important association require further investigation.
心血管事件是非酒精性脂肪性肝病（NAFLD）患者的主要死因，并且很大程度上受遗传因素影响。尽管多项研究表明肿瘤坏死因子-α（TNF-α）基因多态性是NAFLD进展的危险因素，但关于NAFLD患者中这些多态性与冠状动脉疾病（CAD）发生之间关联的报道较少。
本研究旨在评估中国NAFLD患者中TNF-α基因-238和-308位点多态性与发生CAD风险的相关性。
采用聚合酶链反应（PCR）对经B型超声证实的患有CAD（n = 246）、未患CAD（n = 247）的NAFLD患者以及健康对照者（n = 304）进行TNF-α基因238和308位点多态性基因分型。采用生化方法测定血清脂质谱。使用适用于Mac的SPSS统计软件20.0版进行统计分析。
我们发现TNF-α -238位点鸟嘌呤到丙氨酸（GA）多态性以及变异等位基因A的携带者在患CAD和未患CAD的NAFLD患者之间存在显著关联（P < 0.05）。与非携带者相比，TNF-α -238位点A等位基因的携带者在患CAD的NAFLD患者中血清甘油三酯（TG）和低密度脂蛋白（LDL）水平更高（分别为P = 0.025和0.001），在未患CAD的NAFLD患者中TG水平更高（P = 0.017）。
在我们研究的中国汉族人群中，携带TNF-α -238 GA多态性的NAFLD患者发生CAD的风险增加。这种潜在重要关联的潜在机制需要进一步研究。
