Department of Biochemistry; Université de Sherbrooke; Sherbrooke, QC Canada ; ECOGENE-21 and Clinical Research Center and Lipid Clinic; Chicoutimi Hospital; Saguenay, QC Canada.
Department of Surgery; IUCPQ; Quebec, QC Canada.
Adipocyte. 2014 Apr 1;3(2):132-40. doi: 10.4161/adip.28308. Epub 2014 Feb 25.
DNA methylation has been mostly studied in circulating blood cells. Although being readily accessible, metabolically active tissues such as adipose tissue would be more informative for the study of metabolic disorders. However, whether or not the blood DNA methylation profile correlates with that of adipose tissue remains unknown. In this study, DNA methylation patterns of variation at LEP and ADIPOQ gene loci were similar between individual CpGs across the different tissues. We also report that DNA methylation levels at biologically relevant CpGs are correlated between blood, subcutaneous, and visceral adipose tissue, and that these nearby CpGs are associated with LEP and ADIPOQ gene expression in adipose tissues. These results will be highly relevant for future epigenetic studies in metabolic disorders.
DNA 甲基化主要在循环血细胞中进行研究。尽管脂肪组织等代谢活跃的组织易于获取，但对于代谢紊乱的研究来说，它们会提供更有价值的信息。然而，血液 DNA 甲基化谱是否与脂肪组织的甲基化谱相关尚不清楚。在这项研究中，不同组织中个体 CpG 之间的 LEP 和 ADIPOQ 基因座的变异 DNA 甲基化模式相似。我们还报告称，血液、皮下和内脏脂肪组织之间的生物相关 CpG 的 DNA 甲基化水平具有相关性，并且这些附近的 CpG 与脂肪组织中 LEP 和 ADIPOQ 基因的表达相关。这些结果对于代谢紊乱的未来表观遗传学研究将具有重要意义。
