Magee M J, Kripke M L, Ullrich S E
Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Transplantation. 1989 Jun;47(6):1008-13. doi: 10.1097/00007890-198906000-00018.
Previous studies have established that exposure of mice to ultraviolet radiation followed by injection of alloantigen can suppress the induction of delayed hypersensitivity and the rejection of allografts in an antigen-specific manner. In the clinical situation, however, UV irradiation several days prior to transplantation may prove impractical due to the difficulty in predicting when a donor organ will be available. Thus, the purpose of this study was to determine if exposure to UV radiation can suppress the elicitation of the immune response in mice sensitized with alloantigen. The data demonstrate that exposure of mice to UV radiation 1, 3, or 5 days after the injection of alloantigen can significantly suppress the delayed hypersensitivity response to that alloantigen. Present in the spleens of these mice are suppressor T lymphocytes. These suppressor cells are specific for the antigen originally used to sensitize the mice, in that they do not suppress the response to an irrelevant alloantigen. In addition, spleen cells from mice sensitized with alloantigen and exposed to UV radiation 1, 3, or 5 days later are unable to proliferate in response to the alloantigen in a mixed lymphocyte response. These cells do respond to irrelevant third-party cells, demonstrating again the specificity of the suppression. These data demonstrate that exposure of mice in vivo to UV radiation can inhibit the elicitation of the immune response to alloantigen. Since the immunosuppression is specific for the sensitizing antigen, these data suggest that this may provide a novel method of suppressing the immune response to tissue allografts.
以往的研究证实，让小鼠暴露于紫外线辐射后再注射同种异体抗原，能够以抗原特异性的方式抑制迟发型超敏反应的诱导以及同种异体移植物的排斥反应。然而，在临床情况下，由于难以预测何时能获得供体器官，在移植前几天进行紫外线照射可能并不实际。因此，本研究的目的是确定暴露于紫外线辐射是否能抑制用同种异体抗原致敏的小鼠的免疫反应激发。数据表明，在注射同种异体抗原后1、3或5天让小鼠暴露于紫外线辐射，可显著抑制对该同种异体抗原的迟发型超敏反应。这些小鼠的脾脏中存在抑制性T淋巴细胞。这些抑制性细胞对最初用于使小鼠致敏的抗原具有特异性，因为它们不会抑制对无关同种异体抗原的反应。此外，用同种异体抗原致敏且在1、3或5天后暴露于紫外线辐射的小鼠的脾细胞，在混合淋巴细胞反应中无法对同种异体抗原发生增殖反应。这些细胞对无关的第三方细胞有反应，再次证明了抑制作用的特异性。这些数据表明，在体内让小鼠暴露于紫外线辐射可抑制对同种异体抗原的免疫反应激发。由于免疫抑制对致敏抗原具有特异性，这些数据表明这可能提供一种抑制对组织同种异体移植物免疫反应的新方法。
