Liu Yan-Ru, Li Peng-Wei, Suo Jian-Jun, Sun Yan, Zhang Bo-Ai, Lu Hong, Zhu Hong-Can, Zhang Guo-Bin
Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
J Pharm Pharmacol. 2014 Sep;66(9):1265-70. doi: 10.1111/jphp.12261. Epub 2014 Apr 10.
To investigate the protective effect of catalpol on cerebral ischaemia/reperfusion (CI/R) injury in gerbils and further explore the underlying mechanism.
A gerbil model of CI/R was prepared by bilateral common carotid occlusion for 10 min followed by 6 h reperfusion. Catalpol (5, 10 or 20 mg/kg per day) was injected intraperitoneally for 3 days before the carotid occlusion. Stroke index was measured during the reperfusion. The contents of endogenous neuropeptides, endothelin-1 (ET-1) and calcitonin gene-related peptide in plasma were evaluated by radioimmunoassay. Superoxide dismutase (SOD) and malondialdehyde (MDA) in brain tissue homogenate were also examined.
The results showed that catalpol significantly improved the stroke index compared with CI/R control group (P < 0.05 or P < 0.01). Catalpol significantly increased the activity of SOD at the doses of 10 and 20 mg/kg (P ≤ 0.05), decreased the brain MDA content and the plasma level of ET-1 at the doses of 10 and 20 mg/kg (P ≤ 0.01).
These data suggested that the efficacy of catalpol pretreatment on CI/R injury may be attributed to reduction of free radicals and inhibition of lipid peroxidation and ET-1 production.
探讨梓醇对沙土鼠脑缺血/再灌注（CI/R）损伤的保护作用，并进一步探究其潜在机制。
通过双侧颈总动脉闭塞10分钟，随后再灌注6小时制备沙土鼠CI/R模型。在颈动脉闭塞前3天，腹腔注射梓醇（每天5、10或20毫克/千克）。在再灌注期间测量卒中指数。采用放射免疫分析法评估血浆中内源性神经肽、内皮素-1（ET-1）和降钙素基因相关肽的含量。还检测了脑组织匀浆中的超氧化物歧化酶（SOD）和丙二醛（MDA）。
结果表明，与CI/R对照组相比，梓醇显著改善了卒中指数（P<0.05或P<0.01）。梓醇在10和20毫克/千克剂量时显著提高了SOD活性（P≤0.05），在10和20毫克/千克剂量时降低了脑MDA含量和血浆ET-1水平（P≤0.01）。
这些数据表明，梓醇预处理对CI/R损伤的疗效可能归因于自由基的减少、脂质过氧化的抑制和ET-1生成的抑制。
