基于表面增强拉曼散射的中空金芯片一步法检测牛奶中的三聚氰胺。
One-step detection of melamine in milk by hollow gold chip based on surface-enhanced Raman scattering.
作者信息
Guo Zhinan, Cheng Ziyi, Li Ran, Chen Lei, Lv Haiming, Zhao Bing, Choo Jaebum
机构信息
State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China; Department of Bionano Engineering, Hanyang University, Ansan 426-791, South Korea.
State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China.
出版信息
Talanta. 2014 May;122:80-4. doi: 10.1016/j.talanta.2014.01.043. Epub 2014 Jan 31.
A hollow gold (HG) chip with high surface-enhanced Raman scattering (SERS) capability was fabricated and used to monitor the adulteration of milk with melamine. This chip was fabricated with self-assembled hollow gold nanospheres (HGNs) on glass wafers through electrostatic interaction. There are two important advantages for the use of this HG chip as a detection platform. First, HGNs show a strong SERS enhancement from individual particles due to their capability to localize the electromagnetic fields around the pinholes in hollow shells. Second, the HG chip improves the limit of detection through the enrichment effect. The characteristic SERS peak of melamine was used to distinguish it from other kinds of proteins or amino acids, and its intensity was used to monitor the percentage of melamine in milk. With its simple detection procedure (no pretreatment or separation steps), decreased processing time and low detection limit, this HG chip shows a strong potential for broad applications in melamine detection from real samples.
制备了具有高表面增强拉曼散射（SERS）能力的中空金（HG）芯片，并用于监测牛奶中三聚氰胺的掺假情况。该芯片是通过静电相互作用在玻璃晶圆上自组装中空金纳米球（HGNs）制成的。将这种HG芯片用作检测平台有两个重要优点。首先，由于HGNs能够在中空壳层的针孔周围局部化电磁场，单个粒子就表现出很强的SERS增强。其次，HG芯片通过富集效应提高了检测限。利用三聚氰胺的特征SERS峰将其与其他种类的蛋白质或氨基酸区分开来，并利用其强度监测牛奶中三聚氰胺的百分比。该HG芯片检测程序简单（无需预处理或分离步骤）、处理时间缩短且检测限低，在实际样品三聚氰胺检测中具有广阔的应用潜力。
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