Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221, United States.
Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221, United States.
Anal Chim Acta. 2015 May 18;874:49-53. doi: 10.1016/j.aca.2015.03.016. Epub 2015 Mar 11.
Analytical applications of SERS are often more associated with qualitative than quantitative analysis, because of the difficulty in obtaining quantitative SERS results. In this paper we introduce a new strategy to quantitatively measure the SERS signals of analytes based on Au-core/Ag-shell nanoparticles with embedded 4-aminothiophenol as the internal reference. Successful detections of two analytes, Toluidine Blue O in aqueous solution (detection limit of 0.1 μM) and melamine in milk (detection limit of ~5 μM), are demonstrated. The improvement in the linear fitting illustrates that the use of internal reference significantly improves the accuracy of the quantitative SERS measurements. The successful detection of melamine in milk illustrates the versatility of this detection scheme for a wide variety of analytes.
表面增强拉曼散射（SERS）的分析应用通常更多地与定性分析相关，而不是定量分析，因为定量 SERS 结果的获得存在困难。在本文中，我们介绍了一种新策略，该策略基于具有嵌入式 4-巯基苯胺的金核/银壳纳米粒子作为内标，可对分析物的 SERS 信号进行定量测量。成功地检测了两种分析物，水溶液中的甲苯胺蓝 O（检测限为 0.1 μM）和牛奶中的三聚氰胺（检测限约为 5 μM）。线性拟合的改善表明，内标物的使用显著提高了定量 SERS 测量的准确性。在牛奶中成功检测到三聚氰胺说明了该检测方案对于各种分析物的通用性。
