Zhang Ji, Jiang Weiping, Luo Qing, Zhang Xiaoxiao, Guo Qianni, Liu Maili, Zhou Xin
Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, the Chinese Academy of Sciences, Wuhan 430071, PR China.
Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, the Chinese Academy of Sciences, Wuhan 430071, PR China.
Talanta. 2014 May;122:101-5. doi: 10.1016/j.talanta.2014.01.023. Epub 2014 Jan 31.
Although Zn(2+) ions are involved in large numbers of physiopathological processes, non-invasive detection of Zn(2+) ions in opaque biological samples remains a huge challenge. Here, we developed a novel zinc-responsive hyperpolarized (HP) (129)Xe-based NMR molecular sensor. This HP (129)Xe-based NMR molecular sensor was synthesized by attaching 2-(diphenylphosphino) benzenamine as ligand for zinc ions to the xenon-binding supramolecular cage, cryptophane. The (129)Xe NMR spectroscopy of such molecular sensor was shifted up to 6.4 ppm in the presence of Zn(2+) ions, which was nearly four times larger than that of the reported similar sensor. The application of the sensor would benefit low concentration detection by using indirect NMR/MRI method. The response exhibited high sensitivity and selectivity as discriminated from other six potentially competing metal ions. The application of this sensor in the analysis of zinc ions in the rat serum samples was also evaluated. The strategy is generally applicable in developing sensitive and selective sensors for quantitative determination of zinc ions.
尽管锌离子参与了大量的生理病理过程，但在不透明生物样品中对锌离子进行无创检测仍然是一个巨大的挑战。在此，我们开发了一种新型的基于超极化（HP）（129）Xe的锌响应核磁共振分子传感器。这种基于HP（129）Xe的核磁共振分子传感器是通过将作为锌离子配体的2-（二苯基膦基）苯胺连接到氙结合超分子笼穴番上来合成的。在锌离子存在下，这种分子传感器的（129）Xe核磁共振光谱位移高达6.4 ppm，这几乎是已报道的类似传感器的四倍。该传感器的应用将有助于通过间接核磁共振/磁共振成像方法进行低浓度检测。与其他六种潜在竞争金属离子相比，该响应表现出高灵敏度和选择性。还评估了该传感器在大鼠血清样品中锌离子分析中的应用。该策略通常适用于开发用于定量测定锌离子的灵敏且选择性的传感器。
