Yamano M, Hillyard C J, Girgis S, MacIntyre I, Emson P C, Tohyama M
Department of Anatomy II, Osaka University Medical School, Japan.
Brain Res. 1988 Jun 7;451(1-2):179-88. doi: 10.1016/0006-8993(88)90762-7.
An ascending neurone system containing substance P-like immunoreactivity (SPI) from the lateral parabrachial nucleus (PBL) to the central amygdaloid nucleus (AC) was detected. Destruction of the external subdivision of the PBL resulted in a marked ipsilateral reduction of SPI fibres in the AC, which suggests that SPI neurones project mainly ipsilaterally to the AC. This was supported by the findings that injection of biotin-wheatgerm agglutinin into the AC labelled many neurones in the ipsilateral external subdivision of the PBL. Simultaneous staining with antiserum showed that some of these neurones contain SP. Immunohistochemical double-staining revealed that almost all of the SPI neurones in the external subdivision of the PBL contained calcitonin gene-related peptide.
检测到一个从外侧臂旁核（PBL）到中央杏仁核（AC）的含有P物质样免疫反应性（SPI）的上行神经元系统。PBL外部亚区的破坏导致AC中SPI纤维同侧明显减少，这表明SPI神经元主要同侧投射到AC。将生物素-小麦胚芽凝集素注入AC标记了同侧PBL外部亚区的许多神经元，这一发现支持了上述观点。抗血清同时染色显示其中一些神经元含有P物质。免疫组织化学双重染色显示，PBL外部亚区几乎所有的SPI神经元都含有降钙素基因相关肽。
