Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-based Functional Materials and Devices, and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Jiangsu 215123, China.
Nanoscale. 2014 May 21;6(10):5116-20. doi: 10.1039/c3nr06590a.
The interaction between carbon quantum dots (CQDs) and a single living cell was explored in real time. Here, we provide the quantitative data on the permeability of the HeLa cell membrane in the presence of CQDs with different surface functional groups (CQDs terminated with -OH/-COOH (CQD-OH), -PEG (CQD-PEG), and -NH2 (CQD-NH2)). Although these CQDs have very low toxicity towards HeLa cells, they still increase the cell membrane permeability by 8%, 13%, and 19% for CQD-PEG, CQD-OH, and CQD-NH2, respectively, and this kind of permeability was irreversible. These observations are valuable for promoting the bio-applications of carbon nanostructures in living systems.
实时探索了碳量子点（CQDs）与单个活细胞之间的相互作用。在这里，我们提供了有关具有不同表面官能团的 CQDs（用-OH/-COOH（CQD-OH）、-PEG（CQD-PEG）和-NH2（CQD-NH2）封端的 CQDs）存在时 HeLa 细胞膜通透性的定量数据。尽管这些 CQD 对 HeLa 细胞的毒性非常低，但它们仍分别将细胞膜通透性提高了 8%、13%和 19%，对于 CQD-PEG、CQD-OH 和 CQD-NH2，这种通透性是不可逆的。这些观察结果对于促进碳纳米结构在生命系统中的生物应用具有重要意义。
