Pindi Pavan Kumar, R Raja Srinath, Pavankumar Theetha L
Department of Microbiology, Palamuru University, Mahabubnagar, 509001, Andhra Pradesh, India,
J Microbiol. 2014 May;52(5):384-8. doi: 10.1007/s12275-014-3302-2. Epub 2014 Apr 11.
Metagenomics is a magnificent tool to isolate genes from unknown/uncharacterized species and also from organisms that cannot be cultured. In this study, we constructed a metagenomic library from isolated DNA of soil samples collected from Palamuru University campus premises, in Mahabubnagar district of Andhra Pradesh, India. We isolated a novel lipase gene LipHim1, which has an open reading frame of 591 base pairs and encodes ∼23 kDa protein consisting of 196 amino acids. The Lipase LipHim1 showed maximum 32% homology at the protein level with the extracellular Aeromonas hydrophila lipase (Class II, GDSL family) and was significantly different from all other known lipases. The isolated lipase catalyzed the hydrolysis of fatty acid esters of polyoxyethylene sorbitan such as Tween 60. Our results indicate that the isolated lipase gene is novel.
宏基因组学是一种从未知/未表征物种以及无法培养的生物体中分离基因的强大工具。在本研究中，我们从印度安得拉邦马哈布卜纳加尔区帕拉穆鲁大学校园采集的土壤样本中分离出的DNA构建了一个宏基因组文库。我们分离出了一个新的脂肪酶基因LipHim1，其开放阅读框为591个碱基对，编码一个由196个氨基酸组成的约23 kDa的蛋白质。脂肪酶LipHim1在蛋白质水平上与嗜水气单胞菌胞外脂肪酶（II类，GDSL家族）的同源性最高为32%，且与所有其他已知脂肪酶有显著差异。分离出的脂肪酶催化了聚氧乙烯山梨糖醇酐脂肪酸酯（如吐温60）的水解。我们的结果表明，分离出的脂肪酶基因是新的。
