Kooij Viola, Venkatraman Vidya, Tra John, Kirk Jonathan A, Rowell Janelle, Blice-Baum Anna, Cammarato Anthony, Van Eyk Jennifer E
Department of Medicine, Division of Cardiology, The Johns Hopkins University, Baltimore, MD, USA.
Proteomics Clin Appl. 2014 Oct;8(9-10):653-64. doi: 10.1002/prca.201300123. Epub 2014 Jun 25.
Cardiovascular disease is the leading cause of death in the western world. Heart failure is a heterogeneous and complex syndrome, arising from various etiologies, which result in cellular phenotypes that vary from patient to patient. The ability to utilize genetic manipulation and biochemical experimentation in animal models has made them indispensable in the study of this chronic condition. Similarly, proteomics has been helpful for elucidating complicated cellular and molecular phenotypes and has the potential to identify circulating biomarkers and drug targets for therapeutic intervention. In this review, the use of human samples and animal model systems (pig, dog, rat, mouse, zebrafish, and fruit fly) in cardiac research is discussed. Additionally, the protein sequence homology between these species and the extent of conservation at the level of the phospho-proteome in major kinase signaling cascades involved in heart failure are investigated.
心血管疾病是西方世界的主要死因。心力衰竭是一种异质性和复杂性综合征，由多种病因引起，导致患者之间细胞表型各异。在动物模型中利用基因操作和生化实验的能力使其在这种慢性疾病的研究中不可或缺。同样，蛋白质组学有助于阐明复杂的细胞和分子表型，并有可能识别循环生物标志物和治疗干预的药物靶点。在这篇综述中，讨论了人类样本和动物模型系统（猪、狗、大鼠、小鼠、斑马鱼和果蝇）在心脏研究中的应用。此外，还研究了这些物种之间的蛋白质序列同源性以及参与心力衰竭的主要激酶信号级联中磷酸化蛋白质组水平的保守程度。
