Gonçalves Lisandro, Silva-Sousa Yara Teresinha Correa, Raucci Neto Walter, Teixeira Cleonice Silveira, Sousa-Neto Manoel Damião, Alfredo Edson
School of Dentistry, University of Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil.
Microsc Res Tech. 2014 Jun;77(6):446-52. doi: 10.1002/jemt.22365. Epub 2014 Apr 9.
This study assessed the influence of different endodontic chemical substances on the adhesion of the Epiphany SE/Resilon system (with and without resinous solvent) to radicular dentin walls, using the push-out test and scanning electron microscopy (SEM). Forty-eight root canals of human canines were prepared biomechanically with ProTaper rotary files (crown-down technique) and the radicular dentin was treated with either 17% EDTA, 2% chlorhexidine gel (CHX) or 2.5% NaOCl (control). The root canals were filled with Resilon cones and Epiphany SE sealer with and without resinous solvent. Six groups of eight canals each had their roots sectioned transversally to obtain 1-mm thick slices. Data were subjected to statistical analysis by ANOVA and Tukey's tests. The specimens treated with 17% EDTA (1.59 ± 0.91) presented higher bond strength (P < 0.05) than those treated with 2.5% NaOCl (0.93 ± 0.27) and 2% CHX (0.92 ± 0.22). Significantly higher bond strength (P < 0.05) was observed when the Epiphany SE was prepared with (1.37 ± 0.78) than without (0.92 ± 0.33) solvent. Adhesive failures were predominant in all groups. SEM analysis showed greater homogeneity of the filling mass when the solvent was added to the sealer. Treatment of root canal walls with 17% EDTA, and addition of a resinous solvent to Epiphany SE produced the highest adhesion to radicular dentin.
本研究采用推出试验和扫描电子显微镜（SEM），评估了不同牙髓化学物质对Epiphany SE/Resilon系统（含和不含树脂溶剂）与根管牙本质壁黏附力的影响。用人的犬齿的48个根管，采用ProTaper旋转锉进行生物力学预备（冠向下技术），并用17%乙二胺四乙酸（EDTA）、2%氯己定凝胶（CHX）或2.5%次氯酸钠（对照）处理根管牙本质。根管用Resilon牙胶尖和含或不含树脂溶剂的Epiphany SE封闭剂充填。六组，每组8个根管，将其牙根横向切片，得到1毫米厚的薄片。数据采用方差分析和Tukey检验进行统计分析。用17% EDTA处理的标本（1.59±0.91）比用2.5%次氯酸钠（0.93±0.27）和2% CHX（0.92±0.22）处理的标本具有更高的粘结强度（P<0.05）。当制备Epiphany SE时使用溶剂（1.37±0.78）比不使用溶剂（0.92±0.33）时，观察到显著更高的粘结强度（P<0.05）。所有组中粘结失败均占主导。SEM分析表明，当向封闭剂中添加溶剂时，充填材料的均匀性更好。用17% EDTA处理根管壁，并向Epiphany SE中添加树脂溶剂，可产生对根管牙本质的最高黏附力。
