Yavari Hamidreza, Ghasemi Negin, Divband Baharak, Rezaei Yashar, Jabbari Golchin, Payahoo Salar
DDS, MSc, Associate Professor, Department of Endodontics, Dental Faculty, Tabriz University (Medical Sciences), Tabriz, Iran.
DDS, MSc, Assistant Professor, Department of Endodontics, Dental and Periodontal Research Center, Dental Faculty, Tabriz University (Medical Sciences), Tabriz, Iran.
J Clin Exp Dent. 2017 Sep 1;9(9):e1109-e1114. doi: 10.4317/jced.54069. eCollection 2017 Sep.
The aim of this study was to examine and compare the effect of photodynamic therapy and solution containing nano particles Ag/ZnO on adhesion of endodontic sealers to dentinal walls of human root canal.
Ninty single-rooted human teeth were selected and their clinical crown was cut from the cemento-enamel junction zone. Canals were prepared by RaCe rotary system and the smear layer was removed using 17% EDTA and 5.25% NaOCl. Samples were randomly divided into two groups of AH Plus and MTA Fillapex based on the sealer type and each group based on antimicrobial method was divided into two sub-groups of photodynamic therapy and polymer containing nano particles of Ag / ZnO and a control sub-group (N = 15). After obturation of canals with gutta-percha and sealers mentioned, the samples were incubated for a week at a humidity of 95% and 37°C and then 2mm thick discs were prepared from the middle region of roots for Push-out test. The maximum failure force was recorded in newton and converted to MPa. Then, 3 random specimens of each subgroup were evaluated by scanning electron microscopy. Statistical analysis was performed by Two way ANOVA and (<0.05) was considered significant.
The highest (4.84 ± 1.63) and lowest (0.16 ± 0.075) push-out bond strength values were obtained in (AH Plus-PDT) and (MTA Fillapex-Ag/ZnO) respectively. Independent of antimicrobial method, AH Plus bond strength was significantly higher than the MTA Fillapex (<0.001). For both sealers, PDT increased the bond strength significantly (<0.001).
Photodynamic therapy has a positive effect on the bond strength of AH Plus and MTA Fillapex sealers. AH Plus, MTA Fillapex, Nano particles, Photodynamic therapy, Push-out.
本研究的目的是检验并比较光动力疗法和含纳米颗粒Ag/ZnO的溶液对根管封闭剂与人类根管牙本质壁黏附力的影响。
选取90颗单根人牙,从牙骨质-釉质交界区截断临床冠部。采用RaCe旋转系统预备根管,并用17%乙二胺四乙酸(EDTA)和5.25%次氯酸钠(NaOCl)去除玷污层。根据封闭剂类型,样本随机分为AH Plus和MTA Fillapex两组,每组再根据抗菌方法分为光动力疗法和含Ag/ZnO纳米颗粒聚合物两个亚组以及一个对照组(N = 15)。用牙胶和上述封闭剂充填根管后,样本在95%湿度和37°C条件下孵育一周,然后从牙根中部制备2mm厚的切片用于推出试验。记录最大破坏力,单位为牛顿,并换算为兆帕(MPa)。然后,每个亚组随机选取3个样本进行扫描电子显微镜评估。采用双向方差分析进行统计分析,P<0.05认为具有统计学意义。
(AH Plus-光动力疗法)组和(MTA Fillapex-Ag/ZnO)组分别获得最高(4.84±1.63)和最低(0.16±0.075)的推出粘结强度值。不考虑抗菌方法,AH Plus的粘结强度显著高于MTA Fillapex(P<0.001)。对于两种封闭剂,光动力疗法均显著提高了粘结强度(P<0.001)。
光动力疗法对AH Plus和MTA Fillapex封闭剂的粘结强度有积极影响。AH Plus、MTA Fillapex、纳米颗粒、光动力疗法、推出试验