Nishiguchi S, Kuroki T, Otani S, Takeda T, Hirota S, Shimizu Y, Nakajima S, Saito S, Shiomi S, Kobayashi K
Third Department of Internal Medicine, Osaka City University Medical School, Japan.
Hepatology. 1989 Jul;10(1):29-33. doi: 10.1002/hep.1840100107.
Two years or more after 35 patients (29 men and six women) with chronic hepatitis B were treated by interferon, we studied relationships of age, ALT activity, activity of serum DNA polymerase associated with the hepatitis B virus, serum levels of hepatitis B e antigen and activity of 2',5'-oligoadenylate synthetase in peripheral blood mononuclear cells when treatment started in comparison with treatment results. Seventeen patients were given human lymphoblastoid interferon-alpha; the other 18 patients were given interferon-beta. We measured the activity of 2',5'-oligoadenylate synthetase in these mononuclear cells and found the rate of increase in vivo and in vitro; the correlation between the two was r = 0.68. This enzyme activity in the patients who became negative for DNA polymerase after interferon treatment increased more both in vivo and in vitro than in patients who did not became negative. Also, both the in vivo and in vitro activity increased more in patients who became negative for the e antigen after interferon therapy than in those who remained positive. In the first group, interferon was considered to be effective; in the second, ineffective. Of the patients who became negative, some developed e antibodies and some did not; the increase in this enzyme activity in the two groups was not significantly different. The increase in the activity of 2',5'-oligoadenylate synthetase activity could be used to predict the results of interferon treatment and is an index that can be used before treatment to predict the response.
35例慢性乙型肝炎患者（29例男性和6例女性）接受干扰素治疗两年或更长时间后，我们研究了治疗开始时年龄、谷丙转氨酶（ALT）活性、与乙肝病毒相关的血清DNA聚合酶活性、乙肝e抗原血清水平以及外周血单个核细胞中2',5'-寡腺苷酸合成酶活性与治疗结果之间的关系。17例患者给予人淋巴母细胞干扰素-α；另外18例患者给予干扰素-β。我们测定了这些单个核细胞中2',5'-寡腺苷酸合成酶的活性，并发现了其体内和体外的增加率；两者之间的相关性为r = 0.68。干扰素治疗后DNA聚合酶转阴的患者中该酶活性在体内和体外的增加均比未转阴的患者更多。此外，干扰素治疗后e抗原转阴的患者体内和体外活性的增加均比仍为阳性的患者更多。在第一组中，干扰素被认为是有效的；在第二组中则无效。在转阴的患者中，一些产生了e抗体，一些则没有；两组中该酶活性的增加没有显著差异。2',5'-寡腺苷酸合成酶活性的增加可用于预测干扰素治疗的结果，是一种可在治疗前用于预测反应的指标。
