Heathcote J, Kim Y I, Yim C K, LeBrocq J, Read S E
Department of Medicine, University of Toronto, Ontario, Canada.
Hepatology. 1989 Jan;9(1):105-9. doi: 10.1002/hep.1840090118.
Basal levels of the interferon-associated enzyme 2',5'-oligoadenylate synthetase were studied in lymphocytes of 46 patients with acute viral hepatitis and in 46 patients with chronic hepatitis B. Measurement of in vitro production of 2',5'-oligoadenylate synthetase following overnight incubation of lymphocytes with exogenous interferon was used to assess functional capacity of the lymphocyte interferon system. In acute hepatitis patients, an early but transient elevation of 2',5'-oligoadenylate synthetase was observed; the mean level at 1 week was significantly greater than the mean level at 4 weeks after the onset of their illness (p less than 0.01). Serial 2',5'-oligoadenylate synthetase levels did not identify those patients who were to progress to chronic hepatitis. Patients with chronic hepatitis B infection, regardless of background liver histology, generally had normal basal lymphocyte 2',5'-oligoadenylate synthetase levels. In both acute viral hepatitis and chronic hepatitis B, there was an inverse correlation between basal 2',5'-oligoadenylate synthetase level and 2',5'-oligoadenylate synthetase response to overnight incubation with interferon, a finding that suggested a transient down-regulation of interferon responsiveness. These findings provided no support for the hypothesis that there is an inherent or persistently induced deficiency in the interferon system in acute or chronic hepatitis B.
对46例急性病毒性肝炎患者和46例慢性乙型肝炎患者淋巴细胞中的干扰素相关酶2',5'-寡腺苷酸合成酶的基础水平进行了研究。通过检测淋巴细胞与外源性干扰素过夜孵育后2',5'-寡腺苷酸合成酶的体外产生情况，来评估淋巴细胞干扰素系统的功能能力。在急性肝炎患者中，观察到2',5'-寡腺苷酸合成酶早期但短暂升高；发病1周时的平均水平显著高于发病4周时的平均水平（p<0.01）。连续检测2',5'-寡腺苷酸合成酶水平无法识别出那些会进展为慢性肝炎的患者。慢性乙型肝炎感染患者，无论肝脏组织学背景如何，其淋巴细胞2',5'-寡腺苷酸合成酶基础水平通常正常。在急性病毒性肝炎和慢性乙型肝炎中，2',5'-寡腺苷酸合成酶基础水平与干扰素过夜孵育后的2',5'-寡腺苷酸合成酶反应呈负相关，这一发现提示干扰素反应性存在短暂下调。这些发现不支持急性或慢性乙型肝炎中干扰素系统存在内在或持续诱导性缺陷这一假说。
