Scheler Ott, Glynn Barry, Kurg Ants
Department of Biotechnology, IMCB, University of Tartu, Riia 23, Tartu 51010, Estonia.
Expert Rev Mol Diagn. 2014 May;14(4):489-500. doi: 10.1586/14737159.2014.908710. Epub 2014 Apr 11.
There is a growing need for quick and reliable methods for microorganism detection and identification worldwide. Although traditional culture-based technologies are trustworthy and accurate at a relatively low cost, they are also time- and labor-consuming and are limited to culturable bacteria. Those weaknesses have created a necessity for alternative technologies that are capable for faster and more precise bacterial identification from medical, food or environmental samples. The most common current approach is to analyze the nucleic acid component of analyte solution and determine the bacterial composition according to the specific nucleic acid profiles that are present. This review aims to give an up-to-date overview of different nucleic acid target sequences and respective analytical technologies.
全球对快速可靠的微生物检测和鉴定方法的需求日益增长。尽管传统的基于培养的技术值得信赖且成本相对较低，但它们也耗时费力，并且仅限于可培养细菌。这些缺点使得有必要采用替代技术，能够从医学、食品或环境样本中更快、更精确地鉴定细菌。当前最常见的方法是分析分析物溶液的核酸成分，并根据存在的特定核酸谱确定细菌组成。本综述旨在对不同的核酸靶序列和相应的分析技术进行最新概述。
