Dr. B.R. Ambedkar Center for Biomedical Research, University of Delhi, Delhi, India.
Electronics and Communication, Jaypee Institute of Information Technology, Noida, India.
Appl Microbiol Biotechnol. 2022 Sep;106(18):5863-5877. doi: 10.1007/s00253-022-12135-2. Epub 2022 Aug 26.
This mini review focuses on the diagnosis and treatment of virus diseases using Crisper-Cas technology. The present paper describes various strategies involved in diagnosing diseases using Crispr-Cas-based assays. Additionally, CRISPR-Cas systems offer great potential as new therapeutic tools for treating viral infections including HIV, Influenza, and SARS-CoV-2. There are several major challenges to be overcome before this technology can be applied routinely in clinical settings, such as finding a suitable delivery tool, toxicity, and immunogenicity, as well as off-target effects. This review also discusses ways to deal with the challenges associated with Crisper-Cas technology. KEY POINTS: • Crisper technology is being applied to diagnose infectious and non-infectious diseases. • A new generation of CRISPR-Cas-based assays has been developed which detect pathogens within minutes, providing rapid diagnosis of diseases. • Crispr-Cas tools can be used to combat viral infections, specifically HIV, influenza, and SARS-CoV-2.
这篇小型综述聚焦于使用 Crisper-Cas 技术诊断和治疗病毒疾病。本文描述了使用基于 Crispr-Cas 的检测方法诊断疾病的各种策略。此外，CRISPR-Cas 系统作为治疗包括 HIV、流感和 SARS-CoV-2 在内的病毒感染的新治疗工具具有很大的潜力。在这项技术能够常规应用于临床环境之前，还有几个重大挑战需要克服，例如寻找合适的输送工具、毒性和免疫原性以及脱靶效应。本综述还讨论了应对与 Crisper-Cas 技术相关的挑战的方法。
Crisper 技术正被应用于诊断传染性和非传染性疾病。
已经开发出新一代基于 CRISPR-Cas 的检测方法，可以在几分钟内检测病原体，从而快速诊断疾病。
Crispr-Cas 工具可用于对抗病毒感染，特别是 HIV、流感和 SARS-CoV-2。
