Arjomandnejad Motahareh, Muhammadnejad Ahad, Haddadi Mahnaz, Sherkat-Khameneh Narjes, Rismanchi Sanaz, Amanpour Saeid, Muhammadnejad Samad
1)Cancer Models Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences Tehran, Iran. 2)Omid Institute for Advanced Biomodels, Incubation Center for Medical Divices, Tehran University of Medical Sciences, Tehran, Iran.
Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran.
Arch Iran Med. 2014 Apr;17(4):273-7.
Cervical cancer is the seventh most common malignancy in both genders combined and the third most common cancer in women. Despite significant progress in treatments, cervical cancer is not completely curable. Therefore, further research is necessary in this area. Animal models are one of the most practical tools in the field of cancer research. The present study aimed to characterize the growth behavior and surface markers of HeLa cells after heterotopic and systemic inoculation to athymic nude mice.
Ten 6-week old female athymic C57BL/6 nude mice were used in this study. HeLa cells were inoculated into the flank or tail vein. The tumor volume was calculated and growth curves were drawn. Tumor-bearing mice were sacrificed and the lesions obtained after harvesting were analyzed in a pathology lab. Subsequently, one slide per tumor was stained with hematoxylin and eosin (H&E) and other slides were stained immunohistochemically by cytokeratins (CK), vimentin, P53, CD34, and Ki-67.
Tumor take rate, mean doubling time and latency period were 94.4%, 5.29 ± 3.57 days and 15.27 days, respectively. H&E results revealed highly malignant hyperchromatin epithelial cells. Immunohistochemical examination of the heterotopic tumors indicated greater expression of CK and less expression of vimentin compared to the metastatic ones. Sixty percent of cells were P53-positive and more than 80% were Ki-67-positive. CD34 expression indicated the intensity of angiogenesis in tumor.
This study represents a comprehensive description of a HeLa xenograft model for in vivo investigations, enabling researchers to assess new treatments for cervical cancer.
宫颈癌是男女合计第七大常见恶性肿瘤，是女性第三大常见癌症。尽管治疗取得了显著进展，但宫颈癌仍无法完全治愈。因此，该领域有必要进一步开展研究。动物模型是癌症研究领域最实用的工具之一。本研究旨在表征人宫颈癌细胞系（HeLa细胞）异位和全身接种至无胸腺裸鼠后的生长行为及表面标志物。
本研究使用10只6周龄雌性无胸腺C57BL/6裸鼠。将HeLa细胞接种至胁腹或尾静脉。计算肿瘤体积并绘制生长曲线。对荷瘤小鼠实施安乐死，收获后获得的病变组织在病理实验室进行分析。随后，每个肿瘤制作一张苏木精-伊红（H&E）染色切片，其他切片采用细胞角蛋白（CK）、波形蛋白、P53、CD34和Ki-67进行免疫组织化学染色。
肿瘤接种成功率、平均倍增时间和潜伏期分别为94.4%、5.29±3.
