Doctoral School of Health Sciences, Semmelweis University, 1085 Budapest, Hungary.
NNK SSFO National Public Health Center, 1097 Budapest, Hungary.
Molecules. 2023 Jan 20;28(3):1070. doi: 10.3390/molecules28031070.
Xanthohumol is a hop-derived flavonoid that has been widely examined for its health-protecting and antitumorigenic properties, but not yet in a natural beer matrix. The aim of the study was to investigate the antitumorigenic potential of a xanthohumol-enriched beer in vivo. Four groups of 4 × 10 nude mice were formed. Following the injection of HeLa tumorigenic cell lines, the treatment groups were administered a xanthohumol supplementation for 100 days, either dissolved in beer or in an ethanolic solution with the same alcohol strength as beer. The control groups received un-supplemented material. The terminal tumor masses, liver weights, and plasma antioxidant capacities (FRAP and ABTS methods) were measured. For the statistical analysis, a two-way ANOVA test was performed ( < 0.05). There were no statistically significant differences in tumor size between the groups. Xanthohumol did not induce higher levels of plasma antioxidant capacity, neither in beer nor in the water-ethanol matrix. The terminal liver weights were significantly higher in the control group receiving the unsupplemented ethanol solution. Xanthohumol dissolved in beer or in the water-alcohol matrix did not have a protective effect on tumor growth, nor did it have a positive effect on plasma antioxidant capacity either. However, beer with added xanthohumol had a less harmful effect on the liver compared to the supplemented water-ethanol solution. Our results indicate the possible negative countereffect of ethanol; however, further investigations are needed.
黄腐酚是一种源自啤酒花的类黄酮，其具有保护健康和抗肿瘤的特性，已得到广泛研究，但尚未在天然啤酒基质中进行研究。本研究旨在体内研究富含黄腐酚的啤酒的抗肿瘤潜力。将 4 组 4×10 只裸鼠分成 4 组。在注射 HeLa 致瘤细胞系后，用啤酒或与啤酒具有相同酒精强度的乙醇溶液溶解的黄腐酚补充剂对治疗组进行为期 100 天的处理。对照组接受未补充的材料。测量终末肿瘤质量、肝重和血浆抗氧化能力（FRAP 和 ABTS 法）。统计分析采用双向方差分析（ < 0.05）。组间肿瘤大小无统计学差异。黄腐酚既没有在啤酒中也没有在水-乙醇基质中诱导更高水平的血浆抗氧化能力。未补充乙醇溶液的对照组的终末肝重明显更高。溶解在啤酒或水-乙醇基质中的黄腐酚对肿瘤生长没有保护作用，也没有对血浆抗氧化能力产生积极影响。然而，与补充的水-乙醇溶液相比，添加黄腐酚的啤酒对肝脏的影响较小。我们的结果表明，乙醇可能产生负面影响；然而，需要进一步研究。
