Barati Tahereh, Haddadi Mahnaz, Sadeghi Fatemeh, Muhammadnejad Samad, Muhammadnejad Ahad, Heidarian Ronak, Arjomandnejad Motahareh, Amanpour Saeid
Cancer Biology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Research Center for Molecular and Cellular Imaging, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Basic Med Sci. 2018 Jul;21(7):678-681. doi: 10.22038/IJBMS.2018.22938.5835.
Gastric cancer is the third leading cause of cancer-related death worldwide. The overall survival rate of patients is poor because gastric cancers are usually diagnosed at the late stages. Therefore, further research is needed and appropriate research tools are required to develop novel therapeutic approaches.
Eight female athymic nude mice with a background were used in this study. AGS cells were inoculated into the flank. The tumor volumes were calculated and growth curves were drawn. When the volume of the tumors reached 1000 mm3, the animals were humanely euthanized with CO gas. After harvesting, tumors were analyzed with Hematoxylin and Eosin (H&E) and immunohistochemistry (IHC). A pathologist confirmed tumor entity through H&E staining. Tumors were evaluated for expression of HER-2, P53, Ki-67, CD34, cytokeratin 8 (CK8), vimentin, estrogen receptor (ER), and progesterone receptor (PR) utilizing immunohistochemistry.
The tumor take rate was 62.5%, mean doubling time was 40.984 d, and the latency period was 30.62 days. The H&E staining results showed highly malignant hyperchromatin epithelial cells. IHC assessment showed the mutation status of P53 gene. The expression score of the CK8 protein in the tumor cells was +3. Vimentin protein was not expressed and changes in mesenchymal phenotype were not observed. Ki-67 IHC indicated that the proliferation rate was >43% and angiogenesis was defined as high MVD.
The respective AGS xenograft model provides an opportunity to understand the pattern of tumor growth as well as to evaluate new gastric cancer therapies in studies.
胃癌是全球癌症相关死亡的第三大主要原因。由于胃癌通常在晚期才被诊断出来，患者的总体生存率较低。因此，需要进一步开展研究并使用合适的研究工具来开发新的治疗方法。
本研究使用了8只具有特定背景的雌性无胸腺裸鼠。将AGS细胞接种到小鼠侧腹。计算肿瘤体积并绘制生长曲线。当肿瘤体积达到1000立方毫米时，用一氧化碳气体对动物实施安乐死。收获肿瘤后，用苏木精和伊红（H&E）染色及免疫组织化学（IHC）进行分析。病理学家通过H&E染色确认肿瘤实体。利用免疫组织化学评估肿瘤中HER-2、P53、Ki-67、CD34、细胞角蛋白8（CK8）、波形蛋白、雌激素受体（ER）和孕激素受体（PR）的表达情况。
肿瘤接种成功率为62.5%，平均倍增时间为40.984天，潜伏期为30.62天。H&E染色结果显示为高度恶性的深染上皮细胞。免疫组化评估显示了P53基因的突变状态。肿瘤细胞中CK8蛋白的表达评分为+3。波形蛋白未表达，未观察到间充质表型的变化。Ki-67免疫组化显示增殖率>43%，血管生成被定义为高微血管密度（MVD）。
各自的AGS异种移植模型为在研究中了解肿瘤生长模式以及评估新的胃癌治疗方法提供了机会。
