Fu J, Wei P, Zhao C, He C, Yan Z, Hua H
Department of Oral Medicine, Peking University School of Stomatology, Beijing, China.
Oral Dis. 2014 Nov;20(8):815-20. doi: 10.1111/odi.12242. Epub 2014 May 21.
To investigate the antifungal ability of seven over-the-counter mouthwashes against planktonic and sessile Candida albicans and Candida krusei.
The seven mouthwashes studied were Listerine, compound chlorhexidine solution, povidone iodine solution (PV-I), cetylpyridinium chloride solution, Colgate Plax, Crest Prohealth Mouthwash, and NaHCO3 . The antifungal ability of each mouthwash against ATCC90028, ATCC6258, and 10 clinical C. albicans isolates was tested using disk diffusion tests, the broth microdilution method, and biofilm testing with two different XTT-reduction assays. Fluconazole was used as a positive control, and the experiments were performed in triplicate.
Chlorhexidine and cetylpyridinium chloride had the largest inhibition zones for ATCC90028 and ATCC6258 (18.6 ± 3.5 and 19 ± 1.6 mm, respectively). Cetylpyridinium chloride was the most effective at inhibiting all of the planktonic C. albicans strains and ATCC6258 with the minimum inhibitory concentration (MIC). As the maturity of the biofilms increased, the change in sessile cell MIC of the mouthwashes was much smaller than that of fluconazole. For the mature biofilms, chlorhexidine, PV-I, and cetylpyridinium chloride produced the greatest reductions in metabolism (60-80%).
Most of these seven mouthwashes had significant antifungal activity for both planktonic and sessile Candida species.
研究七种非处方漱口水对浮游态和固着态白色念珠菌及克柔念珠菌的抗真菌能力。
所研究的七种漱口水分别为李施德林漱口水、复方氯己定溶液、聚维酮碘溶液（PV - I）、西吡氯铵溶液、高露洁全效护理漱口水、佳洁士健康专家漱口水和碳酸氢钠。采用纸片扩散法、肉汤微量稀释法以及两种不同的XTT还原法进行生物膜检测，测试每种漱口水对ATCC90028、ATCC6258以及10株临床分离的白色念珠菌的抗真菌能力。氟康唑用作阳性对照，实验重复进行三次。
氯己定和西吡氯铵对ATCC90028和ATCC6258的抑菌圈最大（分别为18.6±3.5毫米和19±1.6毫米）。西吡氯铵在抑制所有浮游态白色念珠菌菌株和ATCC6258方面，以最低抑菌浓度（MIC）计最为有效。随着生物膜成熟度增加，漱口水固着细胞MIC的变化远小于氟康唑。对于成熟生物膜，氯己定、PV - I和西吡氯铵使代谢降低的幅度最大（60 - 80%）。
这七种漱口水大多对浮游态和固着态念珠菌均具有显著的抗真菌活性。
