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鲤形目鲤科(某物种)线粒体全基因组的鉴定与分析

Identification and analysis of the complete mitochondrial genome of (Cypriniformes, Cyprinidae).

作者信息

Zou Yuanchao, Chen Mei, Wu Tian, Tian Tian, Wen Zhengyong

机构信息

College of Life sciences, Conservation and Utilization of Fishes resources in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Neijiang Normal University, Neijiang, Sichuan, China.

School of Life Sciences, Southwest University, Chongqing, China.

出版信息

Mitochondrial DNA B Resour. 2018 Feb 26;3(1):270-271. doi: 10.1080/23802359.2018.1443032.

Abstract

is a small-size freshwater ornamental fish. In this study, the complete mitochondrial genome sequence of was first determined. This mitogenome was 16,774 bp in length and contained 13 protein-coding genes (PCGs), 22 tRNA genes, 2 rRNA genes, and 2 main noncoding regions. Most mitochondrial genes were encoded on the H-strand, except for ND6 and eight tRNA genes. The base composition was 28.97% A, 27.72% T, 26.02% C, and 17.29% G, with 56.69% AT, respectively. Phylogenetic tree was constructed based on the nucleotide sequence of 13 PCGs of and closely related 12 species ticks to assess their phylogenic relationship and evolution. These data should be helpful for a better understanding of the mitochondrial genomic diversities and evolution in fish as well as novel genetic markers for studying population genetics and species identification.

摘要

是一种小型淡水观赏鱼。在本研究中,首次测定了其完整的线粒体基因组序列。该线粒体基因组长度为16774 bp,包含13个蛋白质编码基因(PCGs)、22个tRNA基因、2个rRNA基因和2个主要非编码区。除了ND6和8个tRNA基因外,大多数线粒体基因在H链上编码。碱基组成为28.97%A、27.72%T、26.02%C和17.29%G,AT含量分别为56.69%。基于该物种的13个PCGs核苷酸序列以及与之密切相关的12种蜱构建系统发育树,以评估它们的系统发育关系和进化。这些数据有助于更好地了解鱼类线粒体基因组多样性和进化,以及用于研究群体遗传学和物种鉴定的新型遗传标记。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2969/7799968/3b51dde8a84c/TMDN_A_1443032_F0001_C.jpg

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