Pickhard Anja, Gröber Simone, Haug Anna Katharina, Piontek Guido, Wirth Markus, Straßen Ulrich, Rudelius Martina, Reiter Rudolf
Department of Otolaryngology-Head and Neck Surgery, Technical University of Munich, Munich, Germany.
Department of Otolaryngology-Head and Neck Surgery, Technical University of Munich, Munich, Germany.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2014 Jun;117(6):733-42. doi: 10.1016/j.oooo.2014.02.005. Epub 2014 Feb 13.
The purpose of our study was to investigate the expression patterns of cell cycle regulatory proteins and members of the epidermal growth factor receptor (EGFR) signaling pathway in squamous cell carcinoma of the head and neck (HNSCC).
The expression levels of survivin, Bub1 B (budding uninhibited by benzimidazoles 1 homolog beta), PLK-1 (polo-like kinase 1), Ki-67, cyclin D1, p53, EGFR, pMAPK (phosphorylated mitogen-activated protein kinase), pAkt (phosphorylated protein kinase B), and PTEN (phosphatase and tensin homolog) were studied in a series of 180 tumor samples obtained from HNSCC surgical resections, 50 metastatic lymph node samples, and 72 corresponding noncancerous epithelium samples. Protein expression analysis was performed by immunohistochemical staining. The results were correlated with clinicopathologic features and survival data.
Prognostic significance could be found only for the markers survivin and pAkt. Only the marker combination of cyclin D1 and p53 had positive prognosis potential regarding overall survival.
Both pAkt and survivin show a positive correlation with distant metastases and may have utility as predictors of long-term outcomes for patients with HNSCC.
我们研究的目的是调查头颈部鳞状细胞癌（HNSCC）中细胞周期调节蛋白和表皮生长因子受体（EGFR）信号通路成员的表达模式。
在从HNSCC手术切除获得的180个肿瘤样本、50个转移性淋巴结样本和72个相应的非癌上皮样本系列中，研究了生存素、Bub1 B（苯并咪唑不抑制的芽殖1同源物β）、PLK-1（polo样激酶1）、Ki-67、细胞周期蛋白D1、p53、EGFR、pMAPK（磷酸化丝裂原活化蛋白激酶）、pAkt（磷酸化蛋白激酶B）和PTEN（磷酸酶和张力蛋白同源物）的表达水平。通过免疫组织化学染色进行蛋白质表达分析。结果与临床病理特征和生存数据相关。
仅生存素和pAkt这两种标志物具有预后意义。仅细胞周期蛋白D1和p53的标志物组合对总生存具有阳性预后潜力。
pAkt和生存素均与远处转移呈正相关，可能作为HNSCC患者长期预后的预测指标。
