Xiao Tiepeng, Kurita Hiroshi, Li Xiangjun, Qi Fangfang, Shimane Tetsu, Aizawa Hitoshi, Uehara Shinobu
Department of Dentistry and Oral Surgery, Shinshu University School of Medicine, Matsumoto, Japan; Department of Orthodontics, Second Hospital of Hebei Medical University, Shijiazhuang, China.
Department of Dentistry and Oral Surgery, Shinshu University School of Medicine, Matsumoto, Japan.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2014 Jun;117(6):754-9. doi: 10.1016/j.oooo.2014.02.008. Epub 2014 Feb 21.
To assess and compare iodine penetration and glycogen distribution in a vital staining of oral mucosa with iodine solution.
Twenty samples were obtained including both iodine-stained and -unstained mucosa. Intraepithelial iodine was examined using frozen sections. Glycogen distribution was assessed by periodic acid-Schiff staining and transmission electron microscopy.
Iodine accumulation was observed mainly superficially and in the upper and middle thirds of prickle cell layers, with glycogen in almost the whole epithelium except for the para- and basal cell layers. The pattern of iodine and glycogen distribution was classified into 3 types (full-, surround-, and scatter-type). The iodine color was mainly derived from the cells with full- and surround-type distributed glycogen in the upper half of the oral epithelium.
The results of this study suggested that iodine penetrated into nonkeratinized oral epithelium and reacted mainly with intraepithelial glycogen homogeneously distributed in the cytoplasm.
用碘溶液对口腔黏膜进行活体染色，评估并比较碘的渗透情况及糖原分布。
获取20个样本，包括经碘染色和未染色的黏膜。利用冰冻切片检查上皮内碘。通过过碘酸-希夫染色和透射电子显微镜评估糖原分布。
观察到碘主要积聚在棘细胞层的浅层以及上三分之一和中三分之一处，除了旁基底层细胞层外，几乎整个上皮细胞层都有糖原。碘和糖原的分布模式分为3种类型（全染型、周边型和散在型）。碘的颜色主要源于口腔上皮上半部分糖原呈全染型和周边型分布的细胞。
本研究结果表明，碘可渗透至非角化口腔上皮，并主要与细胞质中均匀分布的上皮内糖原发生反应。
