生物测定指导下从突尼斯橄榄叶提取物中分离和鉴定抗棘阿米巴化合物。
Bioassay guided isolation and identification of anti-Acanthamoeba compounds from Tunisian olive leaf extracts.
作者信息
Sifaoui Ines, López-Arencibia Atteneri, Ticona Juan Carlos, Martín-Navarro Carmen M, Reyes-Batlle María, Mejri Mondher, Lorenzo-Morales Jacob, Jiménez Antonio Ignacio, Valladares Basilio, Lopez-Bazzocchi Isabel, Abderabba Manef, Piñero José E
机构信息
Laboratoire Matériaux-Molécules et Applications, IPEST, B.P. 51, 2070 La Marsa, University of Carthage, Tunisia.
University Institute of Tropical Diseases and Public Health, University of La Laguna, Avda Francisco Sanchez s/n, Campus de Anchieta, 38271 la Laguna, Tenerife, Canary Islands, Spain.
出版信息
Exp Parasitol. 2014 Nov;145 Suppl:S111-4. doi: 10.1016/j.exppara.2014.02.018. Epub 2014 Apr 12.
Pathogenic Acanthamoeba strains are causative agents of Granulomatous Amoebic Encephalitis (GAE) and Acanthamoeba keratitis (AK) worldwide. The existence of the cyst stage complicates Acanthamoeba therapy as it is highly resistant to antibiotics and physical agents. The aim of this study was to investigate the activity of Limouni olive leaf cultivar against the trophozoite stage of Acanthamoeba. The ethyl acetate and methanol extracts of this variety were tested against Acanthamoeba castellanii Neff. The ethyl acetate extract of olive leaf was the most active showing an IC50 of 5.11±0.71μg/ml of dry extract. Bio-guided fractionation of this extract was conducted and led to the identification of three active compounds namely oleanolic and maslinic acids and oleuropein which could be used for the development of novel therapeutic approaches against Acanthamoeba infections.
致病性棘阿米巴菌株是全球肉芽肿性阿米巴脑炎（GAE）和棘阿米巴角膜炎（AK）的病原体。囊肿阶段的存在使棘阿米巴治疗变得复杂，因为它对抗生素和物理制剂具有高度抗性。本研究的目的是调查利穆尼橄榄叶品种对棘阿米巴滋养体阶段的活性。该品种的乙酸乙酯和甲醇提取物针对卡氏棘阿米巴Neff进行了测试。橄榄叶的乙酸乙酯提取物活性最强，干提取物的IC50为5.11±0.71μg/ml。对该提取物进行了生物导向分级分离，鉴定出三种活性化合物，即齐墩果酸、山楂酸和橄榄苦苷，它们可用于开发针对棘阿米巴感染的新型治疗方法。
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