一些突尼斯海藻的体外杀阿米巴和抗氧化活性

In vitro amoebicidal and antioxidant activities of some Tunisian seaweeds.

作者信息

Chiboub Olfa, Ktari Leila, Sifaoui Ines, López-Arencibia Atteneri, Reyes-Batlle Maria, Mejri Mondher, Valladares Basilio, Abderrabba Manef, Piñero José E, Lorenzo-Morales Jacob

机构信息

Laboratoire Matériaux-Molécules et Applications, IPEST, B.P 51 2070, La Marsa, University of Carthage, Tunisia; University Institute of Tropical Diseases and Public Health, University of La Laguna, Avda Francisco Sanchez s/n, Campus de Anchieta, 38271 la Laguna Tenerife, Canary Islands, Spain; Laboratory of Blue Biotechnology and Aquatic Bioproducts, National Institute of Marine Sciences and Technologies (INSTM), 28, Rue du 2 mars 1934, 2035, Salammbô, Tunisia.

Laboratory of Blue Biotechnology and Aquatic Bioproducts, National Institute of Marine Sciences and Technologies (INSTM), 28, Rue du 2 mars 1934, 2035, Salammbô, Tunisia.

出版信息

Exp Parasitol. 2017 Dec;183:76-80. doi: 10.1016/j.exppara.2017.10.012. Epub 2017 Nov 2.

DOI:10.1016/j.exppara.2017.10.012

PMID:29102681

Abstract

Free-living amoebae of genus Acanthamoeba are opportunistic pathogens widely distributed in the environment, and are the causative agents of several humans' infections, such as Acanthamoeba keratitis, Granulomatous Amoebic Encephalitis and also disseminated infections. The existence of the cyst stage complicates Acanthamoeba therapy as it is highly resistant to antibiotics and physical agents. All these facts reinforced the necessity to find and develop an effective therapy against Acanthamoeba infections. In the present study, we are interested to several seaweeds species collected from the Tunisian coasts and belonging to the 3 phyla (brown, green and red algae). The aim was to quantify the Total Phenolic Compounds in different organic extract, to evaluate antioxidant capacity (DPPH and ABTS) and to study the antiprotozoal activity against A. castellanii Neff. The parasites have been inhibited by all extracts with an IC ranged from 52,3±1.8 μg/mL for ethyl acetate extract, to 134,6±0.7 μg/mL for the hexanic one for the various species studied.

摘要

棘阿米巴属的自由生活阿米巴是广泛分布于环境中的机会性病原体，是多种人类感染的病原体，如棘阿米巴角膜炎、肉芽肿性阿米巴脑炎以及播散性感染。包囊阶段的存在使棘阿米巴治疗变得复杂，因为它对抗生素和物理因素具有高度抗性。所有这些事实都强化了寻找和开发针对棘阿米巴感染的有效治疗方法的必要性。在本研究中，我们对从突尼斯海岸采集的、属于3个门（褐藻、绿藻和红藻）的几种海藻物种感兴趣。目的是定量不同有机提取物中的总酚化合物，评估抗氧化能力（DPPH和ABTS），并研究对卡氏棘阿米巴Neff的抗原生动物活性。在所研究的各种物种中，所有提取物均对寄生虫有抑制作用，乙酸乙酯提取物的IC50范围为52.3±1.8μg/mL，己烷提取物的IC50范围为134.6±0.7μg/mL。