Ninomiya T, Hosaka M, Higashiyama H, Kurenuma S, Mori M
Department of Oral Surgery, Asahi University, School of Dentistry, Gifu, Japan.
Acta Histochem. 1989;85(2):143-53. doi: 10.1016/S0065-1281(89)80059-5.
8 cases of branchial cleft cysts and 1 case of branchiogenic carcinoma were examined immunohistochemically for detectable keratin, immunoglobulins (IgG, IgA, IgM), carcinoembryonic antigen (CEA), factor VIII related antigen (Factor VIII RGA), and lysozyme. Lectin binding patterns were also determined. Histologically, cystic lining epithelia were classified into stratified squamous epithelium without keratinization, columnar epithelium with or without cilia, or a mixture of both. Almost all of the cases indicated accompanying lymphoid structures with germinal centers. Keratin expression in epithelial cells was slightly positive, and lectin binding affinities in them were similar to those of oral squamous epithelium. CEA was found on the surface border of columnar epithelial cells, but cystic epithelia in most of the cases were devoid of lysozyme. Endothelial cells of capillary vessels showed positive binding by UEA-1 lectin and the presence of factor VIII RAG. In the lymphoid structures, there were scattered strongly positive lysozyme-staining cells as well as a few lymphocytes bearing IgG, IgA, or IgM.
对8例鳃裂囊肿和1例鳃源性癌进行免疫组织化学检查，检测角蛋白、免疫球蛋白（IgG、IgA、IgM）、癌胚抗原（CEA）、因子VIII相关抗原（因子VIII RGA）和溶菌酶。还确定了凝集素结合模式。组织学上，囊壁上皮分为无角化的复层鳞状上皮、有或无纤毛的柱状上皮或两者混合。几乎所有病例均伴有生发中心的淋巴样结构。上皮细胞中的角蛋白表达呈弱阳性，其凝集素结合亲和力与口腔鳞状上皮相似。CEA见于柱状上皮细胞的表面边界，但大多数病例的囊性上皮缺乏溶菌酶。毛细血管内皮细胞显示UEA-1凝集素阳性结合且存在因子VIII RAG。在淋巴样结构中，有散在的溶菌酶染色强阳性细胞以及少数携带IgG、IgA或IgM的淋巴细胞。
