Lidstrom M E
Keck Laboratories, California Institute of Technology, Pasadena 91125.
Antonie Van Leeuwenhoek. 1989;55(1):7-14. doi: 10.1007/BF02309614.
By using molecular probe techniques in combination with activity and expression measurements, it is possible to estimate bacterial populations in nature. This information can be exploited to study a number of important environmental problems. For instance, it will be possible to study ecosystem perturbation and microbial competition, by altering an ecosystem or a laboratory model of an ecosystem, and assessing corresponding changes in key activities and populations. In addition, regulation of activities in the laboratory can be compared to the response of activities and populations in situ, to develop an understanding of the key parameters that control these processes in nature. These types of approaches are important steps for determining the role of microorganisms in geochemical cycling, in both specific habitats and on a global basis.
通过将分子探针技术与活性和表达测量相结合，可以估计自然界中的细菌数量。这些信息可用于研究许多重要的环境问题。例如，通过改变一个生态系统或一个生态系统的实验室模型，并评估关键活性和种群的相应变化，将有可能研究生态系统扰动和微生物竞争。此外，可以将实验室中的活性调节与原位活性和种群的反应进行比较，以了解控制自然界中这些过程的关键参数。这些类型的方法是确定微生物在地球化学循环中的作用的重要步骤，无论是在特定栖息地还是在全球范围内。
