Ferguson R L, Buckley E N, Palumbo A V
Appl Environ Microbiol. 1984 Jan;47(1):49-55. doi: 10.1128/aem.47.1.49-55.1984.
The bacterioplankton community of confined seawater at 25 degrees C changed significantly within 16 h of collection. Confinement increased CFU, total cell number (by epifluorescence microscopy), and average cell volume of bacterioplankton and increased the turnover rate of amino acids in seawater sampled at Frying Pan Shoals, N.C. The bacterioplankton community was characterized by two components: differential doubling times during confinement shifted dominance from bacteria which were nonculturable to bacteria which were culturable on a complex nutrient medium. Culturable cells (especially those of the genera Pseudomonas, Alcaligenes, and Acinetobacter) increased from 0.08% of the total cell number in the seawater immediately after collection to 13% at 16 h and 41% at 32 h of confinement. Differential filtration before confinement indicated that particles passing through a 3.9-microns-, but retained by a 0.2-micron-, pore-size Nuclepore filter may be a major source of primary amines to the confined population. The 3.0-microns filtration increased growth rate and ultimate numbers of culturable cells through the removal of bacterial predators or the release of primary amines from cells damaged during filtration or both.
25摄氏度下封闭海水中的浮游细菌群落，在采集后的16小时内发生了显著变化。封闭环境增加了浮游细菌的菌落形成单位（CFU）、总细胞数（通过落射荧光显微镜观察）和平均细胞体积，并提高了在北卡罗来纳州煎锅浅滩采集的海水中氨基酸的周转率。浮游细菌群落由两个部分组成：封闭期间不同的倍增时间使优势从不可培养的细菌转移到在复杂营养培养基上可培养的细菌。可培养细胞（特别是假单胞菌属、产碱菌属和不动杆菌属的细胞）在采集后立即占海水中总细胞数的0.08%，在封闭16小时时增加到13%，在封闭32小时时增加到41%。封闭前的差异过滤表明，通过孔径为3.9微米但被孔径为0.2微米的核孔滤膜截留的颗粒可能是封闭群体中伯胺的主要来源。3.0微米的过滤通过去除细菌捕食者或从过滤过程中受损的细胞释放伯胺或两者兼而有之，提高了可培养细胞的生长速率和最终数量。
