Morton C R, Hutchison W D, Hendry I A, Duggan A W
Division of Neuroscience, John Curtin School of Medical Research, Australian National University, Canberra.
Brain Res. 1989 May 29;488(1-2):89-96. doi: 10.1016/0006-8993(89)90696-3.
In barbiturate-anaesthetized spinalized cats, antibody microprobes were used to investigate the release of immunoreactive somatostatin (irSS) in the lumbar dorsal horn in response to cutaneous stimuli. In the absence of applied stimulation, a significant basal release of irSS was present in the region of the substantia gelatinosa. Such release was not increased by innocuous or noxious cutaneous mechanical stimuli nor by innocuous thermal stimuli, but was increased by noxious thermal stimulation. The magnitude of this noxious heat-evoked release was estimated by comparing in vivo microprobes with those used to detect known concentrations of somatostatin in vitro. Pairs of microprobes were used to detect simultaneous release of both irSS and immunoreactive substance P in the substantia gelatinosa. The results support the putative role of somatostatin in the spinal transmission of thermal nociceptive information.
在巴比妥麻醉的去脊髓猫中，使用抗体微探针研究腰段背角中免疫反应性生长抑素（irSS）在皮肤刺激下的释放。在未施加刺激时，胶状质区域存在显著的irSS基础释放。这种释放不会因无害或有害的皮肤机械刺激以及无害的热刺激而增加，但会因有害的热刺激而增加。通过将体内微探针与用于检测体外已知浓度生长抑素的微探针进行比较，估算这种有害热诱发释放的幅度。使用双微探针检测胶状质中irSS和免疫反应性P物质的同时释放。结果支持生长抑素在热痛觉信息脊髓传递中的假定作用。
