Morton C R, Hutchison W D
Division of Neuroscience, John Curtin School of Medical Research, Australian National University, Canberra ACT.
Neuroscience. 1989;31(3):807-15. doi: 10.1016/0306-4522(89)90443-0.
In anaesthetized cats, antibody microprobes were used to investigate the release of immunoreactive calcitonin gene-related peptide and galanin in the lower lumbar spinal cord. In the absence of applied stimulation, a basal release of both peptides was detected at the level of the substantia gelatinosa. This release of calcitonin gene-related peptide was not altered by innocuous thermal cutaneous stimulation nor by electrical stimulation of low-threshold myelinated primary afferent fibres, but was increased by noxious thermal or noxious mechanical cutaneous stimuli and by electrical stimulation of unmyelinated primary afferents. A simultaneous release of both calcitonin gene-related peptide and substance P was detected in the substantia gelatinosa region by the use of pairs of microprobes. In contrast, none of the peripheral stimulation procedures increased intraspinal galanin release. The results suggest that the spinal transmission of nociceptive information may involve the simultaneous release and action of several neuropeptides within the superficial layers of the dorsal horn.
在麻醉猫身上，使用抗体微探针研究下腰段脊髓中免疫反应性降钙素基因相关肽（CGRP）和甘丙肽的释放。在未施加刺激时，在脊髓背角胶状质水平检测到这两种肽的基础释放。CGRP的这种释放不受无害热皮肤刺激或低阈值有髓初级传入纤维电刺激的影响，但有害热或有害机械皮肤刺激以及无髓初级传入纤维电刺激可使其增加。通过使用成对的微探针，在胶状质区域检测到CGRP和P物质的同时释放。相比之下，外周刺激程序均未增加脊髓内甘丙肽的释放。结果表明，伤害性信息的脊髓传递可能涉及背角浅层内几种神经肽的同时释放和作用。
