玉米条纹病毒DNA的核苷酸序列。
The nucleotide sequence of maize streak virus DNA.
作者信息
Mullineaux P M, Donson J, Morris-Krsinich B A, Boulton M I, Davies J W
出版信息
EMBO J. 1984 Dec 20;3(13):3063-8. doi: 10.1002/j.1460-2075.1984.tb02258.x.
The nucleotide sequence of the DNA of maize streak virus (MSV) has been determined. The data were accommodated into one DNA circle of 2687 nucleotides, in contrast to previously characterised geminiviruses which have been shown to possess two circles of DNA. Comparison of the nucleotide sequences of the DNA of MSV with those of cassava latent virus (CLV) and tomato golden mosaic virus (TGMV) showed no detectable homology. Analysis of open reading frames revealed seven potential coding regions for proteins of mol. wt. greater than or equal to 10 000, three in the viral (+) sense and four in the complementary (-) sense. The position of likely transcription signals on the MSV DNA sequence would suggest a bidirectional strategy of transcription as proposed for CLV and TGMV. Nine inverted repeat sequences which have a potential of forming hairpin structures of delta G greater than or equal to -14 kcal/mol have been detected. Three of these hairpin structures are in non-coding regions and could be involved in the regulation of transcription and/or replication.
玉米条纹病毒（MSV）DNA的核苷酸序列已被确定。数据整合到一个2687个核苷酸的DNA环中，这与之前已鉴定的双生病毒不同，后者已被证明拥有两个DNA环。将MSV DNA的核苷酸序列与木薯潜隐病毒（CLV）和番茄金色花叶病毒（TGMV）的核苷酸序列进行比较，未发现可检测到的同源性。对开放阅读框的分析揭示了七个可能编码分子量大于或等于10000蛋白质的区域，三个在病毒（+）链上，四个在互补（-）链上。MSV DNA序列上可能的转录信号位置表明，转录策略为双向，这与CLV和TGMV的情况相同。已检测到九个具有形成ΔG大于或等于-14千卡/摩尔发夹结构潜力的反向重复序列。其中三个发夹结构位于非编码区，可能参与转录和/或复制的调控。
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