Gupta Neha, Reddy Kishorekumar, Gnanasekaran Prabu, Zhai Ying, Chakraborty Supriya, Pappu Hanu R
Molecular Virology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Department of Plant Pathology, Washington State University, Pullman, WA, United States.
Front Plant Sci. 2022 Sep 20;13:972386. doi: 10.3389/fpls.2022.972386. eCollection 2022.
Whitefly-transmitted begomoviruses infect and damage a wide range of food, feed, and fiber crops worldwide. Some of these viruses are associated with betasatellite molecules that are known to enhance viral pathogenesis. In this study, we investigated the function of a novel βV1 protein encoded by radish leaf curl betasatellite (RaLCB) by overexpressing the protein using potato virus X (PVX)-based virus vector in . βV1 protein induced lesions on leaves, suggestive of hypersensitive response (HR), indicating cell death. The HR reaction induced by βV1 protein was accompanied by an increased accumulation of reactive oxygen species (ROS), free radicals, and HR-related transcripts. Subcellular localization through confocal microscopy revealed that βV1 protein localizes to the cellular periphery. βV1 was also found to interact with replication enhancer protein (AC3) of helper virus in the nucleus. The current findings suggest that βV1 functions as a protein elicitor and a pathogenicity determinant.
粉虱传播的双生病毒感染并损害全球范围内的多种粮食、饲料和纤维作物。其中一些病毒与已知能增强病毒致病力的β卫星分子有关。在本研究中，我们通过使用基于马铃薯X病毒（PVX）的病毒载体在植物中过表达萝卜叶卷曲β卫星（RaLCB）编码的一种新型βV1蛋白，来研究其功能。βV1蛋白在叶片上诱导形成病斑，提示超敏反应（HR），表明细胞死亡。βV1蛋白诱导的HR反应伴随着活性氧（ROS）、自由基和HR相关转录本积累的增加。通过共聚焦显微镜进行的亚细胞定位显示，βV1蛋白定位于细胞周边。还发现βV1在细胞核中与辅助病毒的复制增强蛋白（AC3）相互作用。目前的研究结果表明，βV1作为一种蛋白激发子和致病决定因素发挥作用。
