MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, People's Republic of China.
Institute of Microbiology and Biochemical Pharmacy, School of Pharmaceutics, Lanzhou University, Lanzhou, People's Republic of China.
Int J Nanomedicine. 2014 Apr 3;9:1699-707. doi: 10.2147/IJN.S58942. eCollection 2014.
Although high melamine (MEL) intake has been proven to cause serious health problems, MEL is sometimes illegally added to milk products and animal feed, arousing serious food safety concerns. A satisfactory method of detecting MEL in onsite or in-home testing is in urgent need of development. This work aimed to explore a rapid, convenient, and cost-effective method of identifying MEL in milk products or other food by colloidal selenium-based lateral flow immunoassay. Colloidal selenium was synthesized by L-ascorbic acid to reduce seleninic acid at room temperature. After conjugation with a monoclonal antibody anti-MEL, a test strip was successfully prepared. The detection limit of the test strip reached 150 μg/kg, 1,000 μg/kg, and 800 μg/kg in liquid milk, milk powder, and animal feed, respectively. No cross-reactions with homologues cyanuric acid, cyanurodiamide, or ammelide were found. Moreover, the MEL test strip can remain stable after storage for 1 year at room temperature. Our results demonstrate that the colloidal selenium MEL test strip can detect MEL in adulterated milk products or animal feed conveniently, rapidly, and sensitively. In contrast with a colloidal gold MEL test strip, the colloidal selenium MEL test strip was easy to prepare and more cost-efficient.
虽然高浓度三聚氰胺（MEL）摄入已被证明会导致严重的健康问题，但 MEL 有时仍被非法添加到奶制品和动物饲料中，引发了严重的食品安全问题。因此，迫切需要开发一种用于现场或家庭检测 MEL 的令人满意的方法。本研究旨在通过基于胶体硒的侧向流动免疫测定法，探索一种快速、便捷且具有成本效益的方法来识别奶制品或其他食品中的 MEL。采用 L-抗坏血酸在室温下还原亚硒酸合成胶体硒。然后将其与抗 MEL 单克隆抗体偶联，成功制备了检测试纸条。该检测试纸条在液态奶、奶粉和动物饲料中的检测限分别达到 150μg/kg、1000μg/kg 和 800μg/kg。与同系物氰尿酸、氰脲酰胺或三聚氰胺没有交叉反应。此外，MEL 检测试纸条在室温下储存 1 年后仍保持稳定。我们的研究结果表明，胶体硒 MEL 检测试纸条可方便、快速、灵敏地检测掺假奶制品或动物饲料中的 MEL。与胶体金 MEL 检测试纸条相比，胶体硒 MEL 检测试纸条易于制备且成本效益更高。
