Department of Physiology, Kyung Hee University School of Medicine, Seoul, Korea.
Department of Urology, Gachon University Gil Medical Center, Gachon University, Incheon, Korea.
Int Neurourol J. 2014 Mar;18(1):16-22. doi: 10.5213/inj.2014.18.1.16. Epub 2014 Mar 31.
Scopolamine is a nonselective muscarinic cholinergic receptor antagonist, which induces impairment of learning ability and memory function. Exercise is known to ameliorate brain disturbance induced by brain injuries. In the present study, we investigated the effect of treadmill exercise on short-term memory in relation to acetylcholinesterase (AChE) expression in the hippocampus, using a scopolamine-induced amnesia model in mice.
To induce amnesia, 1 mg/kg scopolamine hydrobromide was administered intraperitoneally once per day for 14 days. A step-down avoidance test for short-term memory was conducted. AChE histochemistry, immunohistochemistry for collagen IV, and doublecortin were performed.
Short-term memory deteriorated in the mice with scopolamine-induced amnesia, concomitant with enhanced AChE expression and suppression of angiogenesis in the hippocampus. Critically, treadmill exercise ameliorated short-term memory impairment, suppressed AChE expression, and enhanced angiogenesis in the mice with scopolamine-induced amnesia.
Overexpression of AChE is implicated in both brain and renal disease. The findings of our study indicate that treadmill exercise may be of therapeutic value in neurodegenerative and renal diseases by suppressing the effects of AChE expression.
东莨菪碱是一种非选择性毒蕈碱型乙酰胆碱受体拮抗剂，可导致学习能力和记忆功能受损。运动已知可改善脑损伤引起的大脑紊乱。在本研究中，我们使用东莨菪碱诱导的小鼠记忆障碍模型，研究了跑步机运动对短期记忆的影响及其与海马乙酰胆碱酯酶（AChE）表达的关系。
为了诱导记忆障碍，每天腹腔内注射 1mg/kg 氢溴酸东莨菪碱，连续 14 天。进行短期记忆的跳下回避测试。进行 AChE 组织化学、IV 型胶原免疫组织化学和双皮质素染色。
东莨菪碱诱导的记忆障碍小鼠的短期记忆恶化，同时伴有海马 AChE 表达增强和血管生成抑制。重要的是，跑步机运动改善了东莨菪碱诱导的记忆障碍小鼠的短期记忆障碍，抑制了 AChE 表达，并增强了血管生成。
AChE 的过度表达与脑和肾脏疾病有关。我们的研究结果表明，跑步机运动通过抑制 AChE 表达可能对神经退行性疾病和肾脏疾病具有治疗价值。
