Haynes W L, Proia A D, Klintworth G K
Department of Ophthalmology, Duke University Medical Center, Durham, North Carolina.
Invest Ophthalmol Vis Sci. 1989 Jul;30(7):1588-93.
We used computerized image analysis to evaluate quantitatively the ability of topically applied corticosteroids (dexamethasone sodium phosphate, prednisolone acetate), cyclooxygenase inhibitors (flurbiprofen, indomethacin, ketorolac), lipoxygenase inhibitors (REV 5901, esculetin, quercetin), and dual cyclooxygenase/lipoxygenase inhibitors (BW 755C, BW A540C) to reduce corneal neovascularization in the rat induced by silver/potassium nitrate cauterization. Significant decreases in the neovascular response were found with corticosteroids and cyclooxygenase inhibitors. A complete dose-response curve was performed for a representative compound from each class. Dexamethasone was found to be superior to flurbiprofen in its ability to reduce neovascularization in this model, while no significant inhibition was noted with either REV 5901 or BW 755C, even at high doses. We conclude that the corneal angiogenic response in this model can be reduced by inhibition of cyclooxygenase as well as by other mechanisms that are steroid-dependent but are, as yet, poorly defined.
我们使用计算机图像分析技术，定量评估局部应用皮质类固醇（地塞米松磷酸钠、醋酸泼尼松龙）、环氧化酶抑制剂（氟比洛芬、吲哚美辛、酮咯酸）、脂氧化酶抑制剂（REV 5901、七叶亭、槲皮素）以及环氧化酶/脂氧化酶双重抑制剂（BW 755C、BW A540C）对硝酸银/硝酸钾烧灼诱导的大鼠角膜新生血管形成的抑制能力。结果发现，皮质类固醇和环氧化酶抑制剂能显著降低新生血管反应。对每一类中的代表性化合物进行了完整的剂量反应曲线测定。在此模型中，地塞米松在抑制新生血管形成方面的能力优于氟比洛芬，而即使高剂量使用，REV 5901或BW 755C均未观察到显著抑制作用。我们得出结论，该模型中的角膜血管生成反应可通过抑制环氧化酶以及其他依赖类固醇但尚未明确的机制来降低。
