5-氮杂胞苷引起的去甲基化作用导致转基因小鼠中乙型肝炎病毒表面抗原的表达。
Demethylation by 5-azacytidine results in the expression of hepatitis B virus surface antigen in transgenic mice.
作者信息
Araki K, Miyazaki J, Tsurimoto T, Inomoto T, Iwanaga T, Matsubara K, Yamamura K
机构信息
Institute for Medical Genetics, Kumamoto University Medical School.
出版信息
Jpn J Cancer Res. 1989 Apr;80(4):295-8. doi: 10.1111/j.1349-7006.1989.tb02308.x.
Abstract
In 14p3HB transgenic mice, which carry three tandem copies of hepatitis B virus (HBV) DNA, the HBV DNA was significantly methylated and no viral proteins were produced. To analyze the causal relationship between hypermethylation and gene inactivity, 5-azacytidine was injected into the mice to demethylate HBV DNA. When postnatal 14p3HB mice were treated with the drug, hepatitis virus surface antigen was produced in these mice by 3 weeks of age, and the integrated HBV DNA of the liver was less heavily methylated. Our results suggest that injection of 5-azacytidine can be used to efficiently activate a silent transgene such as HBV DNA in transgenic mice.
摘要
在携带三个串联拷贝乙肝病毒（HBV）DNA的14p3HB转基因小鼠中，HBV DNA发生了显著甲基化，且未产生病毒蛋白。为分析高甲基化与基因失活之间的因果关系，向小鼠注射5-氮杂胞苷以使HBV DNA去甲基化。当对出生后的14p3HB小鼠进行药物处理时，这些小鼠在3周龄时产生了肝炎病毒表面抗原，且肝脏中整合的HBV DNA甲基化程度降低。我们的结果表明，注射5-氮杂胞苷可用于有效激活转基因小鼠中如HBV DNA这样的沉默转基因。
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本文引用的文献
1
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Cell. 1981 May;24(2):333-44. doi: 10.1016/0092-8674(81)90323-8.
2
Unusual methylation pattern of the alpha 2 (l) collagen gene.α2（I）型胶原蛋白基因的异常甲基化模式
Cell. 1982 May;29(1):203-10. doi: 10.1016/0092-8674(82)90104-0.
3
Tissue-specific DNA methylation in a cluster of rabbit beta-like globin genes.兔β样珠蛋白基因簇中的组织特异性DNA甲基化
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6634-8. doi: 10.1073/pnas.77.11.6634.
4
Is hypomethylation linked to activation of delta-crystallin genes during lens development?
Nature. 1983;306(5938):88-91. doi: 10.1038/306088a0.
5
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。
Anal Biochem. 1983 Jul 1;132(1):6-13. doi: 10.1016/0003-2697(83)90418-9.
6
DNA methylation in the human gamma delta beta-globin locus in erythroid and nonerythroid tissues.人类红系和非红系组织中γδβ-珠蛋白基因座的DNA甲基化
Cell. 1980 Apr;19(4):947-58. doi: 10.1016/0092-8674(80)90086-0.
7
Cellular differentiation, cytidine analogs and DNA methylation.细胞分化、胞苷类似物与DNA甲基化
Cell. 1980 May;20(1):85-93. doi: 10.1016/0092-8674(80)90237-8.
8
Hepatocellular carcinoma and hepatitis B virus. A prospective study of 22 707 men in Taiwan.肝细胞癌与乙型肝炎病毒。对台湾22707名男性的前瞻性研究。
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9
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