Yamamura K, Araki K, Hino O, Tomita N, Miyazaki J, Matsubara K
Institute for Medical Genetics, Kumamoto University Medical School, Japan.
Gastroenterol Jpn. 1990 Sep;25 Suppl 2:49-52. doi: 10.1007/BF02779928.
We produced transgenic mice by microinjecting a partially duplicated copies of hepatitis B virus (HBV) gene into fertilized eggs of C57BL/6 mice. One mouse was a high producer of HBV surface antigen (HBsAg) and HBV e antigen (HBeAg) in the serum. All offspring carrying HBV DNA were positive for both antigens in the serum. The HBV DNA was expressed in liver- and kidney-specific manner. The normal process of HBV replication, including the packaging of the pregenome 3.5-kb RNA into a nucleocapsid, the reverse-transcription of the complete minus strand DNA, and the release of Dane particles into the serum before the completion of synthesis of plus strand, occurred in the liver of these transgenic mice. These results suggest that the species specificity of HBV infection is not due to the inability to replicate in nonnatural host but to the lack of receptors or factors needed for virus adsorption and internalization. The founder mouse is now 19 months of age but shows no clinical or pathological change, suggesting that HBV itself is not cytopathic.
我们通过将部分重复的乙型肝炎病毒（HBV）基因显微注射到C57BL/6小鼠的受精卵中，培育出了转基因小鼠。有一只小鼠血清中的HBV表面抗原（HBsAg）和HBV e抗原（HBeAg）产量很高。所有携带HBV DNA的后代血清中的这两种抗原均呈阳性。HBV DNA以肝脏和肾脏特异性的方式表达。这些转基因小鼠的肝脏中发生了HBV复制的正常过程，包括将前基因组3.5 kb RNA包装进核衣壳、完整负链DNA的逆转录以及在正链合成完成之前将 Dane 颗粒释放到血清中。这些结果表明，HBV感染的物种特异性并非由于无法在非天然宿主中复制，而是由于缺乏病毒吸附和内化所需的受体或因子。这只奠基小鼠现在19个月大，但未出现临床或病理变化，表明HBV本身没有细胞病变作用。
