利用一种简单的生物测定系统检测新建立的人肝癌细胞系产生的粒细胞集落刺激因子。
Detection of granulocyte colony-stimulating factor produced by a newly established human hepatoma cell line using a simple bioassay system.
作者信息
Tohyama K, Yoshida Y, Kubo A, Sudo T, Moriyama M, Sato H, Uchino H
机构信息
First Division of Internal Medicine, Faculty of Medicine, Kyoto University.
出版信息
Jpn J Cancer Res. 1989 Apr;80(4):335-40. doi: 10.1111/j.1349-7006.1989.tb02316.x.
A colony-stimulating factor(CSF)-producing tumor cell line (KX-87) was established from a patient with hepatocellular carcinoma and marked granulocytosis. This cell line formed tumors on nude mice in high frequency and the mice revealed marked granulocytosis. In clonogenic assays of human bone marrow cells, KX-87 conditioned medium (CM) supported the formation of colonies mainly consisting of neutrophilic granulocytes but had no burst-promoting activity. The molecular weight of the colony-stimulating activity (CSA) in KX-87CM was estimated about 25,000 daltons by gel filtration and a new bioassay system. In principle, a subline of murine hemopoietic cell line NFS-60 was cloned which was dependent on KX-87CM. Then the growth of this subline was examined by a rapid and sensitive colorimetric tetrazolium assay. From these results, it was concluded that the CSA which KX-87 cell line produced was G-CSF.
从一名患有肝细胞癌并伴有明显粒细胞增多症的患者身上建立了一种产生集落刺激因子（CSF）的肿瘤细胞系（KX - 87）。该细胞系在裸鼠身上高频形成肿瘤，且小鼠表现出明显的粒细胞增多症。在人骨髓细胞的克隆形成试验中，KX - 87条件培养基（CM）支持主要由嗜中性粒细胞组成的集落形成，但没有爆式促进活性。通过凝胶过滤和一种新的生物测定系统估计，KX - 87CM中集落刺激活性（CSA）的分子量约为25,000道尔顿。原则上，克隆了依赖于KX - 87CM的小鼠造血细胞系NFS - 60的一个亚系。然后通过快速灵敏的比色四唑盐试验检测该亚系的生长情况。从这些结果得出结论，KX - 87细胞系产生的CSA是粒细胞集落刺激因子（G - CSF）。
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