C 系列神经节苷脂 GT3、GT2 和 GP1c 在大鼠肝脏高尔基体中由同一组糖基转移酶形成，这些酶催化脱唾液酸、a 系列和 b 系列神经节苷脂的生物合成。

The c-series gangliosides GT3, GT2 and GP1c are formed in rat liver Golgi by the same set of glycosyltransferases that catalyse the biosynthesis of asialo-, a- and b-series gangliosides.

作者信息

Iber H, Zacharias C, Sandhoff K

机构信息

Institut für Organische Chemie und Biochemie, Universität Bonn, FRG.

出版信息

Glycobiology. 1992 Apr;2(2):137-42. doi: 10.1093/glycob/2.2.137.

DOI:10.1093/glycob/2.2.137

PMID:1606358

Abstract

Biosynthesis of the c-series gangliosides GT3, GT2 and GP1c was studied in Golgi derived from rat liver. Competition experiments show that the synthesis of ganglioside GT2 (GalNAc beta 1----4-(NeuAc alpha 2----8NeuAc alpha 2----8NeuAc alpha 2----3)Gal- beta 1----4Glc beta 1----1Cer) from GT3 (NeuAc alpha 2----8NeuAc alpha 2----8-NeuAc alpha 2----3Gal beta 1----4Glc beta 1----1Cer) seems to be catalysed by the same N-acetylgalactosaminyl-transferase (GalNAc-T), which converts GM3 (NeuAc alpha 2----3Gal beta 1----4Glc beta 1----1Cer) to GM2 (GalNAc beta 1----4(NeuAc alpha 2----3)Gal beta 1----4Glc beta 1----1Cer). Similar competition experiments suggest moreover that the sialytransferase V (SAT V), which catalyses the synthesis of GT1a (NeuAc alpha 2----8NeuAc alpha 2----3Gal beta 1----3GalNAc beta 1----4- (NeuAc alpha 2----3)-Gal beta 1----4Glc beta 1----1Cer) from GD1a (NeuAc alpha-2----3Gal beta 1----3GalNAc beta 1----4(NeuAc alpha 2----3)Gal beta 1----4Glc beta 1----1-Cer) appears to be identical to the enzyme that catalyses the synthesis of GP1c (NeuAc alpha 2----8NeuAc alpha 2----3Gal beta 1----3-GalNAc beta 1----4(NeuAc alpha 2----8-NeuAc alpha 2----8NeuAc alpha 2----3)Gal beta-1----4Glc beta 1----4Glc beta 1----1Cer) from GQ1c (NeuAc alpha 2----3Gal beta 1----3Gal-NAc beta 1----4 (NeuAc alpha 2----8NeuAc alpha 2----8NeuAc alpha 2----3)Gal beta 1----4-Glc beta 1----1Cer).(ABSTRACT TRUNCATED AT 250 WORDS)

摘要

在源自大鼠肝脏的高尔基体中研究了c系列神经节苷脂GT3、GT2和GP1c的生物合成。竞争实验表明，从GT3（NeuAcα2----8NeuAcα2----8-NeuAcα2----3Galβ1----4Glcβ1----1Cer）合成神经节苷脂GT2（GalNAcβ1----4-(NeuAcα2----8NeuAcα2----8NeuAcα2----3)Gal-β1----4Glcβ1----1Cer）似乎是由相同的N-乙酰半乳糖胺基转移酶（GalNAc-T）催化的，该酶将GM3（NeuAcα2----3Galβ1----4Glcβ1----1Cer）转化为GM2（GalNAcβ1----4(NeuAcα2----3)Galβ1----4Glcβ1----1Cer）。此外，类似的竞争实验表明，催化从GD1a（NeuAcα-2----3Galβ1----3GalNAcβ1----4(NeuAcα2----3)Galβ1----4Glcβ1----1-Cer）合成GT1a（NeuAcα2----8NeuAcα2----3Galβ1----3GalNAcβ1----4- (NeuAcα2----3)-Galβ1----4Glcβ1----1Cer）的唾液酸转移酶V（SAT V）似乎与催化从GQ1c（NeuAcα2----3Galβ1----3Gal-NAcβ1----4 (NeuAcα2----8NeuAcα2----8NeuAcα2----3)Galβ1----4-Glcβ1----1Cer）合成GP1c（NeuAcα2----8NeuAcα2----3Galβ1----3-GalNAcβ1----4(NeuAcα2----8-NeuAcα2----8NeuAcα2----3)Galβ-1----4Glcβ1----4Glcβ1----1Cer）的酶相同。（摘要截选至250字）