Davis J A, Jackson C R, Fedorka-Cray P J, Barrett J B, Brousse J H, Gustafson J, Kucher M
Bacterial Epidemiology and Antimicrobial Resistance Research Unit, USDA-ARS, Richard B. Russell Research Center, Athens, GA, USA.
Lett Appl Microbiol. 2014 Jul;59(1):1-8. doi: 10.1111/lam.12254. Epub 2014 Apr 15.
Antimicrobial-resistant staphylococci have been associated with wounded or ill companion animals, but little is known about the prevalence of resistant staphylococci among healthy animals. In this study, 276 healthy dogs and cats from veterinary clinics were tested for the presence of antimicrobial-resistant Staphylococcus spp. Isolates were tested for antimicrobial susceptibility and the presence of select resistance genes, and typed using Pulsed-Field Gel Electrophoresis (PFGE). Staphylococcus aureus and Staphylococcus pseudintermedius were also characterized using multilocus sequence typing (MLST), spa typing and SCCmec typing. Approximately 5% (14/276) of the animals were positive by enrichment for five species of staphylococci [Staph. aureus (n = 11), Staph. pseudintermedius (n = 4), Staphylococcus sciuri (n = 6), Staphylococcus simulans (n = 1) and Staphylococcus warneri (n = 1)]. Seventy-eight per cent (18/23) of staphylococci were resistant to oxacillin and also multidrug resistant (resistance to ≥ 2 antimicrobials). All Staph. aureus isolates were mecA+ and blaZ+, SCCmec type II, spa type t002, ST5 and clonal using PFGE. Staphylococcus pseudintermedius were SCCmec type IV or V, spa type t06 and ST170; two of the isolates were pvl(+) . These results suggest that healthy companion animals may be a reservoir of multidrug-resistant staphylococci, which may be transferred to owners and others who handle companion animals.
In this study, antimicrobial-resistant coagulase-negative and coagulase-positive staphylococci were isolated from various body sites on healthy dogs and cats. Resistance to 14 antimicrobials was observed including resistance to oxacillin; the majority of staphylococci were also multidrug resistant. Results from this study suggest that healthy dogs and cats may act as reservoirs of antimicrobial-resistant bacteria that may be transferred to people by simple interaction with the animals. Such carriage poses an underlying risk of infection, which should be considered during handling of healthy dogs and cats by pet owners and veterinary personnel.
耐抗菌药物的葡萄球菌与受伤或患病的伴侣动物有关，但对于健康动物中耐抗菌药物葡萄球菌的流行情况知之甚少。在本研究中，对来自兽医诊所的276只健康犬猫进行了耐抗菌药物葡萄球菌属的检测。对分离株进行了抗菌药物敏感性和特定耐药基因的检测，并使用脉冲场凝胶电泳（PFGE）进行分型。还使用多位点序列分型（MLST）、spa分型和SCCmec分型对金黄色葡萄球菌和中间葡萄球菌进行了特征分析。通过富集培养，约5%（14/276）的动物对五种葡萄球菌呈阳性[金黄色葡萄球菌（n = 11）、中间葡萄球菌（n = 4）、松鼠葡萄球菌（n = 6）、模仿葡萄球菌（n = 1）和沃氏葡萄球菌（n = 1）]。78%（18/23）的葡萄球菌对苯唑西林耐药且多重耐药（对≥2种抗菌药物耐药）。所有金黄色葡萄球菌分离株均为mecA+和blaZ+，SCCmec II型，spa t002型，ST5型且通过PFGE分型为克隆型。中间葡萄球菌为SCCmec IV型或V型，spa t06型，ST170型；其中两株分离株为pvl(+)。这些结果表明，健康的伴侣动物可能是多重耐药葡萄球菌的储存宿主，这些葡萄球菌可能会传播给主人和其他接触伴侣动物的人。
在本研究中，从健康犬猫的不同身体部位分离出了耐抗菌药物的凝固酶阴性和凝固酶阳性葡萄球菌。观察到对14种抗菌药物耐药，包括对苯唑西林耐药；大多数葡萄球菌也多重耐药。本研究结果表明，健康的犬猫可能作为耐抗菌药物细菌的储存宿主，通过与动物的简单接触就可能传播给人。这种携带状态构成了潜在的感染风险，宠物主人和兽医人员在处理健康犬猫时应予以考虑。
