Arefin Kazi Shams Al, Rana Eaftekhar Ahmed, Islam Md Saiful, Hossain Belayet, Rahman Md Ashiqur, Barua Himel
Department of Microbiology and Veterinary Public Health, Chattogram Veterinary and Animal Sciences University, Khulshi, Chattogram 4225, Bangladesh.
One Health Institute, Chattogram Veterinary and Animal Sciences University, Khulshi, Chattogram 4225, Bangladesh.
Vet Med Int. 2025 Aug 21;2025:4879266. doi: 10.1155/vmi/4879266. eCollection 2025.
Coagulase-positive staphylococci, notably and , are common pathogens that are frequently associated with suppurative infections in companion animals. This cross-sectional study aimed to investigate the molecular prevalence, antimicrobial resistance profiles, and associated risk factors of and carriage in cats. Standard bacteriological procedures as well as the and genes specific PCR were performed to isolate and identify the and . Methicillin-resistant (MRSA) and methicillin-resistant (MRSP) were identified by detecting the A gene. A total of 251 swab samples were collected from different body sites of 80 cats. The results showed that 24 (30%) and 5 (6.25%) cats carried and , respectively. isolates exhibited the highest resistance to penicillin (94.29%) and ampicillin (91.5%), while 60% of isolates were resistant to ampicillin, erythromycin, and ciprofloxacin. Thirty-two (91%) isolates and two (40%) isolates demonstrated resistance to ≥ 3 classes of antimicrobials, indicating multidrug resistance (MDR). The prevalence of MRSA was 11.43%, with none of the isolates carrying the gene and neither nor harboring the gene. Multivariable logistic regression identified the previous use of antibiotics as a risk factor for colonization in cats (odds ratio [OR] 3.84; 95% CI: 1.23-11.95). Cats with otitis externa (OR 46; 95% CI: 3.2-660.4) and steroid use (OR 46; 95% CI: 3.2-660.4) were potential risk factors for carriage. The circulation of MDR isolates, including the detection of MRSA, poses a significant concern for the clinical management of companion animals.
凝固酶阳性葡萄球菌，尤其是金黄色葡萄球菌和中间葡萄球菌，是常见病原体，常与伴侣动物的化脓性感染有关。这项横断面研究旨在调查猫体内金黄色葡萄球菌和中间葡萄球菌携带情况的分子流行率、抗菌药物耐药谱及相关危险因素。采用标准细菌学程序以及金黄色葡萄球菌和中间葡萄球菌特异性基因的聚合酶链反应（PCR）来分离和鉴定金黄色葡萄球菌和中间葡萄球菌。通过检测mecA基因来鉴定耐甲氧西林金黄色葡萄球菌（MRSA）和耐甲氧西林中间葡萄球菌（MRSP）。从80只猫的不同身体部位共采集了251份拭子样本。结果显示，分别有24只（30%）和5只（6.25%）猫携带金黄色葡萄球菌和中间葡萄球菌。金黄色葡萄球菌分离株对青霉素（94.29%）和氨苄西林（91.5%）的耐药性最高，而60%的中间葡萄球菌分离株对氨苄西林、红霉素和环丙沙星耐药。32株（91%）金黄色葡萄球菌分离株和2株（40%）中间葡萄球菌分离株对≥3类抗菌药物耐药，表明存在多重耐药（MDR）。MRSA的流行率为11.43%，金黄色葡萄球菌分离株均未携带mecA基因，中间葡萄球菌既未携带mecA基因也未携带PVL基因。多变量逻辑回归分析确定，先前使用抗生素是猫感染金黄色葡萄球菌的一个危险因素（比值比[OR]3.
