Sarkar Biswatrish, Kumar Dhananjay, Sasmal Dinakar, Mukhopadhyay Kunal
a Department of Pharmaceutical Sciences , Birla Institute of Technology , Mesra, Ranchi 835215 , Jharkhand , India.
Nat Prod Res. 2014;28(17):1393-8. doi: 10.1080/14786419.2014.904309. Epub 2014 Apr 14.
Anthocyanin extracts (AEs) from Ocimum tenuiflorum (leaf), Hibiscus rosa-sinensis (petal) and Hibiscus sabdariffa (calyx) were investigated and compared for in vitro antioxidant activity and DNA damage protective property. Total phenolic content (TPC) and total anthocyanin content (TAC) of the AEs were determined and the major anthocyanins were characterised. In vitro antioxidant activities were assessed by ferric-reducing antioxidant power (FRAP) assay, 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical-scavenging activity, 2-deoxy-D-ribose degradation assay and lipid peroxidation assay. The protective property of the AEs was also examined against oxidative DNA damage by H2O2 and UV using pUC19 plasmid. All the AEs particularly those from O. tenuiflorum demonstrated efficient antioxidant activity and protected DNA from damage. Strong correlation between antioxidant capacity and TPC and TAC was observed. Significant correlation between antioxidant capacity and TPC and TAC ascertained that phenolics and anthocyanins were the major contributors of antioxidant activity.
对罗勒（叶）、朱槿（花瓣）和玫瑰茄（花萼）中的花青素提取物（AE）进行了研究和比较，以考察其体外抗氧化活性和DNA损伤保护特性。测定了AE的总酚含量（TPC）和总花青素含量（TAC），并对主要花青素进行了表征。通过铁还原抗氧化能力（FRAP）测定、2,2-二苯基-1-苦基肼（DPPH）自由基清除活性、2-脱氧-D-核糖降解测定和脂质过氧化测定来评估体外抗氧化活性。还使用pUC19质粒检测了AE对H2O2和紫外线诱导的氧化性DNA损伤的保护特性。所有AE，尤其是罗勒中的AE，均表现出高效的抗氧化活性，并能保护DNA免受损伤。观察到抗氧化能力与TPC和TAC之间存在强相关性。抗氧化能力与TPC和TAC之间的显著相关性表明，酚类和花青素是抗氧化活性的主要贡献者。
