Kapepula Paulin Mutwale, Kabamba Ngombe Nadege, Tshisekedi Tshibangu Pascal, Tsumbu César, Franck Thierry, Mouithys-Mickalad Ange, Mumba Dieudonné, Tshala-Katumbay Désiré, Serteyn Didier, Tits Monique, Angenot Luc, Kalenda Pascal Dibungi T, Frédérich Michel
a Laboratory of Pharmacognosy , Center for Interdisciplinary Research on Medicines (CIRM), University of Liège , Liège , Belgium.
b Faculty of Pharmaceutical Sciences , Centre d'Etudes des Substances Naturelles d'Origine Végétale (CESNOV), University of Kinshasa , Kinshasa , Democratic Republic of Congo.
Nat Prod Res. 2017 Dec;31(24):2885-2892. doi: 10.1080/14786419.2017.1305382. Epub 2017 Mar 21.
Methanolic and dichloromethane extracts from the leaves of Congolese Hibiscus species were characterised by chromatographic and spectroscopic techniques and their in vitro biochemical activities against ROS production were evaluated in cellular models and on an enzyme, myeloperoxidase (MPO), involved in inflammation. Hibiscus acetosella has a chemical fingerprint different from Hibiscus cannabinus and Hibiscus sabdariffa both having similar fingerprints. Major compounds were polyphenols, represented mainly by caffeoyl-hydroxycitric acid for H. acetosella and neochlorogenic acid for the two other species. All extracts displayed high cellular antioxidant activity with IC values ranging from 0.5 to 3 μg mL using lucigenin on neutrophils. Dichloromethane extracts showed more efficient effects on extracellular ROS production and MPO activity. Antioxidant and anti-inflammatory activities of caffeoyl-hydroxycitric acid were significantly higher than those of neochlorogenic acid. The bioactivities of Hibiscus species were positively correlated with their phytochemical content and could justify their use as local nutraceutical resources and medicines.
采用色谱和光谱技术对刚果芙蓉属植物叶片的甲醇提取物和二氯甲烷提取物进行了表征，并在细胞模型中以及在参与炎症反应的髓过氧化物酶（MPO）上评估了它们对活性氧生成的体外生化活性。红叶槿的化学指纹图谱与大麻槿和玫瑰茄不同，后两者指纹图谱相似。主要化合物为多酚类，红叶槿中主要为咖啡酰羟基柠檬酸，另外两种则主要为新绿原酸。使用光泽精对中性粒细胞进行检测时，所有提取物均表现出较高的细胞抗氧化活性，IC值范围为0.5至3μg/mL。二氯甲烷提取物对细胞外活性氧生成和MPO活性的影响更为显著。咖啡酰羟基柠檬酸的抗氧化和抗炎活性明显高于新绿原酸。芙蓉属植物的生物活性与其植物化学成分呈正相关，这可以证明它们作为当地营养保健品资源和药物的用途是合理的。
