1INSERM U844, University Hospital Saint Eloi, Montpellier, France.
Int Rev Immunol. 2014 Nov-Dec;33(6):470-89. doi: 10.3109/08830185.2014.902453. Epub 2014 Apr 14.
Classical and non-classical monocytes are two well-defined subsets of monocytes displaying distinct roles. They differentially express numerous genes relevant to their primary role. Using five independent transcriptomic microarray datasets, we ruled out several inconsistent genes and identified common genes consistently overexpressed either in classical or non-classical monocytes. One hundred and eight genes were significantly increased in classical monocytes and are involved in bacterial defense, inflammation and atherosclerosis. Whereas the 74 genes overexpressed in non-classical monocytes are involved in cytoskeletal dynamics and invasive properties for enhanced motility and infiltration. These signatures unravel the biological functions of monocyte subsets. HIGHLIGHTS We compared five transcriptomic GEO datasets of human monocyte subsets. 108 genes in classical and 74 genes in non-classical monocytes are upregulated. Upregulated genes in classical monocytes support anti-bacterial and inflammatory responses. Upregulated genes in non-classical monocytes support patrolling and infiltration functions.
经典单核细胞和非经典单核细胞是两种定义明确的单核细胞亚群，它们具有不同的作用。它们差异表达许多与其主要作用相关的基因。使用五个独立的转录组微阵列数据集，我们排除了几个不一致的基因，并鉴定出在经典单核细胞或非经典单核细胞中一致过表达的常见基因。108 个基因在经典单核细胞中显著上调，参与细菌防御、炎症和动脉粥样硬化。而非经典单核细胞中过表达的 74 个基因参与细胞骨架动力学和侵袭特性，以增强运动和浸润能力。这些特征揭示了单核细胞亚群的生物学功能。
我们比较了五个人类单核细胞亚群的转录组 GEO 数据集。经典单核细胞中有 108 个基因和非经典单核细胞中有 74 个基因上调。经典单核细胞中上调的基因支持抗细菌和炎症反应。非经典单核细胞中上调的基因支持巡逻和浸润功能。
